Next Article in Journal
Salt-Stress Response Mechanisms Using de Novo Transcriptome Sequencing of Salt-Tolerant and Sensitive Corchorus spp. Genotypes
Next Article in Special Issue
Comparative Genomics of Non-TNL Disease Resistance Genes from Six Plant Species
Previous Article in Journal
Predicting Variation of DNA Shape Preferences in Protein-DNA Interaction in Cancer Cells with a New Biophysical Model
Previous Article in Special Issue
Mapping of QTLs for Seed Phorbol Esters, a Toxic Chemical in Jatropha curcas (L.)
Article Menu
Issue 9 (September) cover image

Export Article

Open AccessArticle
Genes 2017, 8(9), 231; doi:10.3390/genes8090231

Transcription Factors Responding to Pb Stress in Maize

1
Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China
2
Animal Nutrition Research Institute, Sichuan Agricultural University, Chengdu 611130, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 3 August 2017 / Revised: 7 September 2017 / Accepted: 15 September 2017 / Published: 18 September 2017
(This article belongs to the Special Issue Plant Genomics and Epigenomics for Trait Improvement)
View Full-Text   |   Download PDF [5693 KB, uploaded 18 September 2017]   |  

Abstract

Pb can damage the physiological function of human organs by entering the human body via food-chain enrichment. Revealing the mechanisms of maize tolerance to Pb is critical for preventing this. In this study, a Pb-tolerant maize inbred line, 178, was used to analyse transcription factors (TFs) expressed under Pb stress based on RNA sequencing data. A total of 464 genes expressed in control check (CK) or Pb treatment samples were annotated as TFs. Among them, 262 differentially expressed transcription factors (DETs) were identified that responded to Pb treatment. Furthermore, the DETs were classified into 4 classes according to their expression patterns, and 17, 12 and 2 DETs were significantly annotated to plant hormone signal transduction, basal transcription factors and base excision repair, respectively. Seventeen DETs were found to participate in the plant hormone signal transduction pathway, where basic leucine zippers (bZIPs) were the most significantly enriched TFs, with 12 members involved. We further obtained 5 Arabidopsis transfer DNA (T-DNA) mutants for 6 of the maize bZIPs, among which the mutants atbzip20 and atbzip47, representing ZmbZIP54 and ZmbZIP107, showed obviously inhibited growth of roots and above-ground parts, compared with wild type. Five highly Pb-tolerant and 5 highly Pb-sensitive in maize lines were subjected to DNA polymorphism and expression level analysis of ZmbZIP54 and ZmbZIP107. The results suggested that differences in bZIPs expression partially accounted for the differences in Pb-tolerance among the maize lines. Our results contribute to the understanding of the molecular regulation mechanisms of TFs in maize under Pb stress. View Full-Text
Keywords: transcription factor; maize; stress; validation transcription factor; maize; stress; validation
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zhang, Y.; Ge, F.; Hou, F.; Sun, W.; Zheng, Q.; Zhang, X.; Ma, L.; Fu, J.; He, X.; Peng, H.; Pan, G.; Shen, Y. Transcription Factors Responding to Pb Stress in Maize. Genes 2017, 8, 231.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top