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Genes 2017, 8(9), 220; doi:10.3390/genes8090220

Zebrafish Xenograft: An Evolutionary Experiment in Tumour Biology

Department of Biology, University of New Brunswick, Fredericton, NB E3B 5A3, Canada
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Academic Editor: Laura Sánchez
Received: 2 August 2017 / Revised: 27 August 2017 / Accepted: 29 August 2017 / Published: 5 September 2017
(This article belongs to the Special Issue Zebrafish: The Key for Cancer Treatment)
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Abstract

Though the cancer research community has used mouse xenografts for decades more than zebrafish xenografts, zebrafish have much to offer: they are cheap, easy to work with, and the embryonic model is relatively easy to use in high-throughput assays. Zebrafish can be imaged live, allowing us to observe cellular and molecular processes in vivo in real time. Opponents dismiss the zebrafish model due to the evolutionary distance between zebrafish and humans, as compared to mice, but proponents argue for the zebrafish xenograft’s superiority to cell culture systems and its advantages in imaging. This review places the zebrafish xenograft in the context of current views on cancer and gives an overview of how several aspects of this evolutionary disease can be addressed in the zebrafish model. Zebrafish are missing homologs of some human proteins and (of particular interest) several members of the matrix metalloproteinase (MMP) family of proteases, which are known for their importance in tumour biology. This review draws attention to the implicit evolutionary experiment taking place when the molecular ecology of the xenograft host is significantly different than that of the donor. View Full-Text
Keywords: xenograft; zebrafish; extracellular matrix; matrix metalloproteinases; MMPs xenograft; zebrafish; extracellular matrix; matrix metalloproteinases; MMPs
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wyatt, R.A.; Trieu, N.P.V.; Crawford, B.D. Zebrafish Xenograft: An Evolutionary Experiment in Tumour Biology. Genes 2017, 8, 220.

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