Next Article in Journal
Regulation and Modulation of Human DNA Polymerase δ Activity and Function
Previous Article in Journal
Boron Tolerance in Aspergillus nidulans Is Sustained by the SltA Pathway Through the SLC-Family Transporters SbtA and SbtB
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Genes 2017, 8(7), 189; doi:10.3390/genes8070189

Developmental Expression of HSP60 and HSP10 in the Coilia nasus Testis during Upstream Spawning Migration

1
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Shanshui Road 9, Wuxi 214000, Jiangsu, China
2
Scientific Observing and Experimental Station of Fishery Resources and Environment in the Lower Reaches of the Yangtze River, Ministry of Agriculture, Xuejiali 69, Wuxi 214000, Jiangsu, China
*
Author to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Received: 2 June 2017 / Revised: 17 July 2017 / Accepted: 19 July 2017 / Published: 21 July 2017
(This article belongs to the Section Molecular Genetics)
View Full-Text   |   Download PDF [6367 KB, uploaded 25 July 2017]   |  

Abstract

Heat shock protein 60 (HSP60) and heat shock protein 10 (HSP10) are important chaperones, which have been proven to have essential roles in mediating the correct folding of nuclear encoded proteins imported to mitochondria. Mitochondria are known as the power house of the cell, with which it produces energy and respires aerobically. In this regard, the obtained HSP60 and HSP10 have typical characteristics of the HSP60/10 family signature. Their mRNA transcripts detected were highest during the developmental phase (in April), while the lowest levels were found in the resting phase (after spawning in late July). Additionally, the strongest immunolabeling positive signals were found in the primary spermatocyte, with lower positive staining in secondary sperm cells, and a weak or absent level in the mature sperm. At the electron microscopic level, immunogold particles were localized in the mitochondrial matrix. Data indicated that HSP10 and HSP60 were inducible and functional in the Coilia nasus testis development and migration process, suggesting their essential roles in this process. The results also indicated that HSP60 may be one indicator of properly working mitochondrial import and refolding in the fish testis. This study also provides an expanded perspective on the role of heat shock protein families in spawning migration biology. View Full-Text
Keywords: Coilia nasus; Spawning Migration; Heat shock protein 60; Heat shock protein 10 Coilia nasus; Spawning Migration; Heat shock protein 60; Heat shock protein 10
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Fang, D.-A.; Zhou, Y.-F.; Zhang, M.-Y.; Xu, D.-P.; Liu, K.; Duan, J.-R. Developmental Expression of HSP60 and HSP10 in the Coilia nasus Testis during Upstream Spawning Migration. Genes 2017, 8, 189.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top