R Loops in the Regulation of Antibody Gene Diversification
AbstractFor nearly three decades, R loops have been closely linked with class switch recombination (CSR), the process that generates antibody isotypes and that occurs via a complex cascade initiated by transcription-coupled mutagenesis in switch recombination sequences. R loops form during transcription of switch recombination sequences in vitro and in vivo, and there is solid evidence that R loops are required for efficient class switching. The classical model of R loops posits that they boost mutation rates by generating stable and long tracts of single-stranded DNA that serve as the substrate for activation induced deaminase (AID), the enzyme that initiates the CSR reaction cascade by co-transcriptionally mutating ssDNA in switch recombination sequences. Though logical and compelling, this model has not been supported by in vivo evidence. Indeed, several reports suggest that R loops may not be involved in recruiting AID activity to switch regions, meaning that R loops probably serve other unanticipated roles in CSR. Here, I review the key findings in this field to date and propose hypotheses that could help towards elucidating the precise function of R loops in CSR. View Full-Text
- Supplementary File 1:
Supplementary (PDF, 72 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Pavri, R. R Loops in the Regulation of Antibody Gene Diversification. Genes 2017, 8, 154.
Pavri R. R Loops in the Regulation of Antibody Gene Diversification. Genes. 2017; 8(6):154.Chicago/Turabian Style
Pavri, Rushad. 2017. "R Loops in the Regulation of Antibody Gene Diversification." Genes 8, no. 6: 154.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.