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Genes 2017, 8(2), 84; doi:10.3390/genes8020084

A Cross-Species Gene Expression Marker-Based Genetic Map and QTL Analysis in Bambara Groundnut

1
Biotechnology Research Centre, School of Biosciences, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia
2
Crops For the Future, Jalan Broga, Semenyih 43500, Selangor Darul Ehsan, Malaysia
3
Plant and Crop Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Leics, Loughborough LE12 5RD, UK
*
Author to whom correspondence should be addressed.
Academic Editor: J. Peter W. Young
Received: 5 December 2016 / Revised: 9 January 2017 / Accepted: 11 January 2017 / Published: 22 February 2017
(This article belongs to the Section Plant Genetics and Genomics)
View Full-Text   |   Download PDF [2531 KB, uploaded 22 February 2017]   |  

Abstract

Bambara groundnut (Vigna subterranea (L.) Verdc.) is an underutilised legume crop, which has long been recognised as a protein-rich and drought-tolerant crop, used extensively in Sub-Saharan Africa. The aim of the study was to identify quantitative trait loci (QTL) involved in agronomic and drought-related traits using an expression marker-based genetic map based on major crop resources developed in soybean. The gene expression markers (GEMs) were generated at the (unmasked) probe-pair level after cross-hybridisation of bambara groundnut leaf RNA to the Affymetrix Soybean Genome GeneChip. A total of 753 markers grouped at an LOD (Logarithm of odds) of three, with 527 markers mapped into linkage groups. From this initial map, a spaced expression marker-based genetic map consisting of 13 linkage groups containing 218 GEMs, spanning 982.7 cM (centimorgan) of the bambara groundnut genome, was developed. Of the QTL detected, 46% were detected in both control and drought treatment populations, suggesting that they are the result of intrinsic trait differences between the parental lines used to construct the cross, with 31% detected in only one of the conditions. The present GEM map in bambara groundnut provides one technically feasible route for the translation of information and resources from major and model plant species to underutilised and resource-poor crops. View Full-Text
Keywords: Affymetrix GeneChip; cross-hybridisation; gene expression markers; quantitative trait loci Affymetrix GeneChip; cross-hybridisation; gene expression markers; quantitative trait loci
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Chai, H.H.; Ho, W.K.; Graham, N.; May, S.; Massawe, F.; Mayes, S. A Cross-Species Gene Expression Marker-Based Genetic Map and QTL Analysis in Bambara Groundnut. Genes 2017, 8, 84.

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