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Genes 2017, 8(11), 336; doi:10.3390/genes8110336

Assessment of Bifidobacterium Species Using groEL Gene on the Basis of Illumina MiSeq High-Throughput Sequencing

1,2,†
,
1,2,†
,
1,2
,
1,2
,
1,2,3
,
1,2,3,* and 1,2,3,4
1
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
2
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
3
National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, China
4
Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 31 October 2017 / Accepted: 15 November 2017 / Published: 21 November 2017
(This article belongs to the Section Microbial Genetics and Genomics)
View Full-Text   |   Download PDF [3356 KB, uploaded 22 November 2017]   |  

Abstract

The next-generation high-throughput sequencing techniques have introduced a new way to assess the gut’s microbial diversity on the basis of 16S rRNA gene-based microbiota analysis. However, the precise appraisal of the biodiversity of Bifidobacterium species within the gut remains a challenging task because of the limited resolving power of the 16S rRNA gene in different species. The groEL gene, a protein-coding gene, evolves quickly and thus is useful for differentiating bifidobacteria. Here, we designed a Bifidobacterium-specific primer pair which targets a hypervariable sequence region within the groEL gene that is suitable for precise taxonomic identification and detection of all recognized species of the genus Bifidobacterium so far. The results showed that the novel designed primer set can specifically differentiate Bifidobacterium species from non-bifidobacteria, and as low as 104 cells of Bifidobacterium species can be detected using the novel designed primer set on the basis of Illumina Miseq high-throughput sequencing. We also developed a novel protocol to assess the diversity of Bifidobacterium species in both human and rat feces through high-throughput sequencing technologies using groEL gene as a discriminative marker. View Full-Text
Keywords: Bifidobacterium; groEL; species level; biodiversity; MiSeq high-throughput sequencing Bifidobacterium; groEL; species level; biodiversity; MiSeq high-throughput sequencing
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MDPI and ACS Style

Hu, L.; Lu, W.; Wang, L.; Pan, M.; Zhang, H.; Zhao, J.; Chen, W. Assessment of Bifidobacterium Species Using groEL Gene on the Basis of Illumina MiSeq High-Throughput Sequencing. Genes 2017, 8, 336.

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