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Genes 2017, 8(11), 325; doi:10.3390/genes8110325

Development of Novel Polymorphic EST-SSR Markers in Bailinggu (Pleurotus tuoliensis) for Crossbreeding

1
Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China
2
China National GeneBank, Environmental Genomics, Beijing Genomics Institute, Shenzhen 518083, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Juan Francisco Martín
Received: 16 October 2017 / Revised: 16 October 2017 / Accepted: 8 November 2017 / Published: 17 November 2017
(This article belongs to the Section Microbial Genetics and Genomics)
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Abstract

Identification of monokaryons and their mating types and discrimination of hybrid offspring are key steps for the crossbreeding of Pleurotus tuoliensis (Bailinggu). However, conventional crossbreeding methods are troublesome and time consuming. Using RNA-seq technology, we developed new expressed sequence tag-simple sequence repeat (EST-SSR) markers for Bailinggu to easily and rapidly identify monokaryons and their mating types, genetic diversity and hybrid offspring. We identified 1110 potential EST-based SSR loci from a newly-sequenced Bailinggu transcriptome and then randomly selected 100 EST-SSRs for further validation. Results showed that 39, 43 and 34 novel EST-SSR markers successfully identified monokaryons from their parent dikaryons, differentiated two different mating types and discriminated F1 and F2 hybrid offspring, respectively. Furthermore, a total of 86 alleles were detected in 37 monokaryons using 18 highly informative EST-SSRs. The observed number of alleles per locus ranged from three to seven. Cluster analysis revealed that these monokaryons have a relatively high level of genetic diversity. Transfer rates of the EST-SSRs in the monokaryons of closely-related species Pleurotus eryngii var. ferulae and Pleurotus ostreatus were 72% and 64%, respectively. Therefore, our study provides new SSR markers and an efficient method to enhance the crossbreeding of Bailinggu and closely-related species. View Full-Text
Keywords: Pleurotus tuoliensis; expressed sequence tag-simple sequence repeat; monokaryon; genetic diversity; transferability Pleurotus tuoliensis; expressed sequence tag-simple sequence repeat; monokaryon; genetic diversity; transferability
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Dai, Y.; Su, W.; Yang, C.; Song, B.; Li, Y.; Fu, Y. Development of Novel Polymorphic EST-SSR Markers in Bailinggu (Pleurotus tuoliensis) for Crossbreeding. Genes 2017, 8, 325.

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