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Genes 2017, 8(11), 319; doi:10.3390/genes8110319

Human Organ Tissue Identification by Targeted RNA Deep Sequencing to Aid the Investigation of Traumatic Injury

1
National Center for Forensic Science, University of Central Florida, Orlando, FL 32816, USA
2
Department of Chemistry, University of Central Florida, Orlando, FL 32816, USA
*
Author to whom correspondence should be addressed.
Received: 17 October 2017 / Revised: 3 November 2017 / Accepted: 6 November 2017 / Published: 10 November 2017
(This article belongs to the Special Issue Forensic Genomics)
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Abstract

Molecular analysis of the RNA transcriptome from a putative tissue fragment should permit the assignment of its source to a specific organ, since each will exhibit a unique pattern of gene expression. Determination of the organ source of tissues from crime scenes may aid in shootings and other investigations. We have developed a prototype massively parallel sequencing (MPS) mRNA profiling assay for organ tissue identification that is designed to definitively identify 10 organ/tissue types using a targeted panel of 46 mRNA biomarkers. The identifiable organs and tissues include brain, lung, liver, heart, kidney, intestine, stomach, skeletal muscle, adipose, and trachea. The biomarkers were chosen after iterative specificity testing of numerous candidate genes in various tissue types. The assay is very specific, with little cross-reactivity with non-targeted tissue, and can detect RNA mixtures from different tissues. We also demonstrate the ability of the assay to successful identify the tissue source of origin using a single blind study. View Full-Text
Keywords: massively parallel sequencing; human organ tissue; mRNA; tissue identification; forensic science massively parallel sequencing; human organ tissue; mRNA; tissue identification; forensic science
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Hanson, E.; Ballantyne, J. Human Organ Tissue Identification by Targeted RNA Deep Sequencing to Aid the Investigation of Traumatic Injury. Genes 2017, 8, 319.

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