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Genes 2017, 8(10), 271; doi:10.3390/genes8100271

Effects of Conjugated Linoleic Acid Supplementation on the Expression Profile of miRNAs in Porcine Adipose Tissue

1,†
,
1,2,†
,
1
,
1
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3,* , 1,2,* and 1,2,*
1
Chongqing Academy of Animal Sciences, Chongqing 402460, China
2
Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing Key Laboratory of Pig Industry Sciences, Chongqing 402460, China
3
The Department of Animal Husbandry, Rongchang Campus, Southwest University, Rongchang, Chongqing 402460, China
These two authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 14 September 2017 / Revised: 21 September 2017 / Accepted: 21 September 2017 / Published: 13 October 2017
(This article belongs to the Section Animal Genetics and Genomics)
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Abstract

Conjugated linoleic acids (CLAs) play a major role in adipocyte differentiation and lipid metabolism in animals. MicroRNAs (miRNAs) appear to be involved in many biological processes in adipose tissue. However, the specific influence on miRNAs by CLA supplementation in porcine adipose tissue remains unclear. Thus, we continuously added 1.5% CLA to the pig diet from the embryo stage to the finishing period and conducted a high-throughput sequencing approach to analyse the changes in adipose tissue miRNAs. We identified 283 known porcine miRNAs, and 14 miRNAs were differentially expressed in response to CLA treatment. A Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the targets of the 14 differentially expressed miRNAs were involved in the Wnt signalling pathway. The CLA treatment downregulated the gene expression of PPARγ, C/EBPα, FAS, and FATP1 in both subcutaneous and abdominal fat tissues; the analysis showed that ssc-miR-21 expression was significantly correlated with PPARγ expression (p<0.05), and speculated that ssc-miR-21 might influence adipogenesis through PPARγ. In conclusion, our study analysed the miRNA profiles in porcine adipose tissues by CLA treatment, and demonstrated that miRNAs are important regulators of fat lipogenesis. This study provides valuable information for the molecular regulatory mechanism of CLA on adipose tissue. View Full-Text
Keywords: miRNA; conjugated linoleic acids; pig; adipose tissue; differential expression miRNA; conjugated linoleic acids; pig; adipose tissue; differential expression
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MDPI and ACS Style

Wang, Q.; Qi, R.; Liu, H.; Wang, J.; Huang, W.; Yang, F.; Huang, J. Effects of Conjugated Linoleic Acid Supplementation on the Expression Profile of miRNAs in Porcine Adipose Tissue. Genes 2017, 8, 271.

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