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Genes 2017, 8(1), 28; doi:10.3390/genes8010028

Transgenic Tobacco Expressing the TAT-Helicokinin I-CpTI Fusion Protein Show Increased Resistance and Toxicity to Helicoverpa armigera (Lepidoptera: Noctuidae)

1
Key Laboratory of Forest Protection, State Forestry Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China
2
College of Forestry, Henan University of Science and Technology, Luoyang 471003, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Received: 2 December 2016 / Revised: 26 December 2016 / Accepted: 5 January 2017 / Published: 12 January 2017
(This article belongs to the Section Molecular Genetics)
View Full-Text   |   Download PDF [1857 KB, uploaded 12 January 2017]   |  

Abstract

Insect kinins were shown to have diuretic activity, inhibit weight gain, and have antifeedant activity in insects. In order to study the potential of the TAT-fusion approach to deliver diuretic peptides per os to pest insects, the HezK I peptide from Helicoverpa zea, as a representative of the kinin family, was selected. The fusion gene TAT-HezK I was designed and was used to transform tobacco plants. As a means to further improve the stability of TAT-HezK I, a fusion protein incorporating HezK I, transactivator of transcription (TAT), and the cowpea trypsin inhibitor (CpTI) was also designed. Finally, the toxicity of the different tobacco transgenic strains toward Helicoverpa armigera was compared. The results demonstrated that TAT-HezK I had high toxicity against insects via transgenic expression of the peptide in planta and intake through larval feeding. The toxicity of the fusion TAT-HezK I and CpTI was higher than the CpTI single gene in transgenic tobacco, and the fusion TAT-HezK I and CpTI further enhanced the stability and bioavailability of agents in oral administration. Our research helps in targeting new genes for improving herbivore tolerance in transgenic plant breeding. View Full-Text
Keywords: helicokinin I; cowpea trypsin inhibitor (CpTI); transactivator of transcription (TAT); protein transduction domain (PTD); Helicoverpa armigera helicokinin I; cowpea trypsin inhibitor (CpTI); transactivator of transcription (TAT); protein transduction domain (PTD); Helicoverpa armigera
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhou, Z.; Li, Y.; Yuan, C.; Zhang, Y.; Qu, L. Transgenic Tobacco Expressing the TAT-Helicokinin I-CpTI Fusion Protein Show Increased Resistance and Toxicity to Helicoverpa armigera (Lepidoptera: Noctuidae). Genes 2017, 8, 28.

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