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Genes 2016, 7(6), 26; doi:10.3390/genes7060026

A Multi-Step miRNA-mRNA Regulatory Network Construction Approach Identifies Gene Signatures Associated with Endometrioid Endometrial Carcinoma

1
Department of Pathology, Zhujiang Hospital, Southern Medical University, Guangzhou 510515, China
2
Department of Pathology, the Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510150, China
3
Department of Gynaecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou 510150, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Nora L. Nock
Received: 5 April 2016 / Revised: 16 May 2016 / Accepted: 24 May 2016 / Published: 2 June 2016
(This article belongs to the Section Human Genomics and Genetic Diseases)
View Full-Text   |   Download PDF [220 KB, uploaded 2 June 2016]

Abstract

We aimed to identify endometrioid endometrial carcinoma (EEC)-related gene signatures using a multi-step miRNA-mRNA regulatory network construction approach. Pathway analysis showed that 61 genes were enriched on many carcinoma-related pathways. Among the 14 highest scoring gene signatures, six genes had been previously shown to be endometrial carcinoma. By qRT-PCR and next generation sequencing, we found that a gene signature (CPEB1) was significantly down-regulated in EEC tissues, which may be caused by hsa-miR-183-5p up-regulation. In addition, our literature surveys suggested that CPEB1 may play an important role in EEC pathogenesis by regulating the EMT/p53 pathway. The miRNA-mRNA network is worthy of further investigation with respect to the regulatory mechanisms of miRNAs in EEC. CPEB1 appeared to be a tumor suppressor in EEC. Our results provided valuable guidance for the functional study at the cellular level, as well as the EEC mouse models. View Full-Text
Keywords: CPEB1; endometrial carcinoma; miRNA targets; protein-protein interaction network; EMT; p53 CPEB1; endometrial carcinoma; miRNA targets; protein-protein interaction network; EMT; p53
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Xiong, H.; Li, Q.; Chen, R.; Liu, S.; Lin, Q.; Xiong, Z.; Jiang, Q.; Guo, L. A Multi-Step miRNA-mRNA Regulatory Network Construction Approach Identifies Gene Signatures Associated with Endometrioid Endometrial Carcinoma. Genes 2016, 7, 26.

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