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Erratum published on 2 February 2017, see Genes 2017, 8(2), 58.

Open AccessArticle
Genes 2016, 7(10), 78; doi:10.3390/genes7100078

Cloning and Expression Analysis of MEP Pathway Enzyme-encoding Genes in Osmanthus fragrans

1
College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China
2
Key Laboratory of Forest Genetics & Gene Engineering of the Ministry of Education, Nanjing Forestry University, Nanjing 210037, China
3
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
4
College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China
*
Author to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Received: 24 June 2016 / Revised: 15 September 2016 / Accepted: 22 September 2016 / Published: 29 September 2016
(This article belongs to the Section Molecular Genetics)
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Abstract

The 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway is responsible for the biosynthesis of many crucial secondary metabolites, such as carotenoids, monoterpenes, plastoquinone, and tocopherols. In this study, we isolated and identified 10 MEP pathway genes in the important aromatic plant sweet osmanthus (Osmanthus fragrans). Multiple sequence alignments revealed that 10 MEP pathway genes shared high identities with other reported proteins. The genes showed distinctive expression profiles in various tissues, or at different flower stages and diel time points. The qRT-PCR results demonstrated that these genes were highly expressed in inflorescences, which suggested a tissue-specific transcript pattern. Our results also showed that OfDXS1, OfDXS2, and OfHDR1 had a clear diurnal oscillation pattern. The isolation and expression analysis provides a strong foundation for further research on the MEP pathway involved in gene function and molecular evolution, and improves our understanding of the molecular mechanism underlying this pathway in plants. View Full-Text
Keywords: Osmanthus fragrans; MEP pathway; tissue-specific; flower development; diel oscillations Osmanthus fragrans; MEP pathway; tissue-specific; flower development; diel oscillations
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Xu, C.; Li, H.; Yang, X.; Gu, C.; Mu, H.; Yue, Y.; Wang, L. Cloning and Expression Analysis of MEP Pathway Enzyme-encoding Genes in Osmanthus fragrans. Genes 2016, 7, 78.

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