Cloning and Expression Analysis of MEP Pathway Enzyme-encoding Genes in Osmanthus fragrans
AbstractThe 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway is responsible for the biosynthesis of many crucial secondary metabolites, such as carotenoids, monoterpenes, plastoquinone, and tocopherols. In this study, we isolated and identified 10 MEP pathway genes in the important aromatic plant sweet osmanthus (Osmanthus fragrans). Multiple sequence alignments revealed that 10 MEP pathway genes shared high identities with other reported proteins. The genes showed distinctive expression profiles in various tissues, or at different flower stages and diel time points. The qRT-PCR results demonstrated that these genes were highly expressed in inflorescences, which suggested a tissue-specific transcript pattern. Our results also showed that OfDXS1, OfDXS2, and OfHDR1 had a clear diurnal oscillation pattern. The isolation and expression analysis provides a strong foundation for further research on the MEP pathway involved in gene function and molecular evolution, and improves our understanding of the molecular mechanism underlying this pathway in plants. View Full-Text
- Supplementary File 1:
Supplementary (DOC, 2754 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Xu, C.; Li, H.; Yang, X.; Gu, C.; Mu, H.; Yue, Y.; Wang, L. Cloning and Expression Analysis of MEP Pathway Enzyme-encoding Genes in Osmanthus fragrans. Genes 2016, 7, 78.
Xu C, Li H, Yang X, Gu C, Mu H, Yue Y, Wang L. Cloning and Expression Analysis of MEP Pathway Enzyme-encoding Genes in Osmanthus fragrans. Genes. 2016; 7(10):78.Chicago/Turabian Style
Xu, Chen; Li, Huogen; Yang, Xiulian; Gu, Chunsun; Mu, Hongna; Yue, Yuanzheng; Wang, Lianggui. 2016. "Cloning and Expression Analysis of MEP Pathway Enzyme-encoding Genes in Osmanthus fragrans." Genes 7, no. 10: 78.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.