Decreased Expression of KIFC1 in Human Testes with Globozoospermic Defects
AbstractGlobozoospermia is a rare (prevalence of <0.1%) but severe male infertility condition. In our previous study, we found that robust KIFC1 immunostaining was detected in the human elongating/elongated spermatids during human acrosomogenesis. However, the relationship between the decreased expression of KIFC1 and human globozoospermia remains largely unknown. Testicular biopsies of 30 globozoospermia and 30 obstructive azoospermia patients who underwent infertility evaluation and treatment were utilized in this study. Reverse transcriptase polymerase chain reaction (RT-PCR), Western blots, immunohistochemistry, an in vivo model, and intratesticular injection of small inhibitory RNA (siRNA) against the Kifc1 gene were employed, and sperm abnormalities were evaluated by hematoxylin and eosin (H&E) staining and immunocytochemistry. We revealed that the testicular level of KIFC1 mRNA in globozoospermia was significantly reduced compared with that in obstructive azoospermia, and the KIFC1 protein was barely detectable in testicular specimens in 30% (9 of 30) of patients with globozoospermia. Furthermore, knockdown of the Kifc1 gene in mice increased the percentage of sperm with globozoospermic defects (26.5%). Decreased KIFC1 expression was mainly observed in the testes of patients with globozoospermia at the spermatid stage, which may be useful for counseling and management of such patients. View Full-Text
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Zhi, E.; Li, P.; Chen, H.; Xu, P.; Zhu, X.; Zhu, Z.; He, Z.; Li, Z. Decreased Expression of KIFC1 in Human Testes with Globozoospermic Defects. Genes 2016, 7, 75.
Zhi E, Li P, Chen H, Xu P, Zhu X, Zhu Z, He Z, Li Z. Decreased Expression of KIFC1 in Human Testes with Globozoospermic Defects. Genes. 2016; 7(10):75.Chicago/Turabian Style
Zhi, Erlei; Li, Peng; Chen, Huixing; Xu, Peng; Zhu, Xiaobin; Zhu, Zijue; He, Zuping; Li, Zheng. 2016. "Decreased Expression of KIFC1 in Human Testes with Globozoospermic Defects." Genes 7, no. 10: 75.
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