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Genes 2016, 7(10), 75; doi:10.3390/genes7100075

Decreased Expression of KIFC1 in Human Testes with Globozoospermic Defects

1,2,†
,
1,2,†
,
1,2
,
3
,
4
,
1,2
,
5,* and 1,2,*
1
Department of Andrology, Center for Men’s Health, Urologic Medical Center, Shanghai General Hospital, Shanghai Jiao Tong University, 100 Haining Rd, Shanghai 200080, China
2
Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Jiao Tong University, 100 Haining Rd, Shanghai 200080, China
3
Oriental Medical Group Jinghua Hospital of Sheng yang, 156 Nanjing Rd, Shengyang 110000, China
4
IVF Unit, Department of Obstetrics and Gynecology, Ruijin Hospital Affiliated to Shanghai Jiao Tong University, 197 Ruijin Er Rd, Shanghai 200025, China
5
State Key Laboratory of Oncogenes and Related Genes, Renji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University,160 Pujian Road, Shanghai 200127, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Received: 25 July 2016 / Revised: 13 September 2016 / Accepted: 18 September 2016 / Published: 27 September 2016
(This article belongs to the Section Molecular Genetics)
View Full-Text   |   Download PDF [4748 KB, uploaded 27 September 2016]   |  

Abstract

Globozoospermia is a rare (prevalence of <0.1%) but severe male infertility condition. In our previous study, we found that robust KIFC1 immunostaining was detected in the human elongating/elongated spermatids during human acrosomogenesis. However, the relationship between the decreased expression of KIFC1 and human globozoospermia remains largely unknown. Testicular biopsies of 30 globozoospermia and 30 obstructive azoospermia patients who underwent infertility evaluation and treatment were utilized in this study. Reverse transcriptase polymerase chain reaction (RT-PCR), Western blots, immunohistochemistry, an in vivo model, and intratesticular injection of small inhibitory RNA (siRNA) against the Kifc1 gene were employed, and sperm abnormalities were evaluated by hematoxylin and eosin (H&E) staining and immunocytochemistry. We revealed that the testicular level of KIFC1 mRNA in globozoospermia was significantly reduced compared with that in obstructive azoospermia, and the KIFC1 protein was barely detectable in testicular specimens in 30% (9 of 30) of patients with globozoospermia. Furthermore, knockdown of the Kifc1 gene in mice increased the percentage of sperm with globozoospermic defects (26.5%). Decreased KIFC1 expression was mainly observed in the testes of patients with globozoospermia at the spermatid stage, which may be useful for counseling and management of such patients. View Full-Text
Keywords: KIFC1; globozoospermic defects; male infertility; kinesin14 family KIFC1; globozoospermic defects; male infertility; kinesin14 family
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MDPI and ACS Style

Zhi, E.; Li, P.; Chen, H.; Xu, P.; Zhu, X.; Zhu, Z.; He, Z.; Li, Z. Decreased Expression of KIFC1 in Human Testes with Globozoospermic Defects. Genes 2016, 7, 75.

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