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Genes 2016, 7(10), 72; doi:10.3390/genes7100072

Transition and Transversion Mutations Are Biased towards GC in Transposons of Chilo suppressalis (Lepidoptera: Pyralidae)

1
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
2
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Received: 8 July 2016 / Revised: 13 September 2016 / Accepted: 18 September 2016 / Published: 24 September 2016
(This article belongs to the Section Molecular Genetics)
View Full-Text   |   Download PDF [2298 KB, uploaded 24 September 2016]   |  

Abstract

Transposons are often regulated by their hosts, and as a result, there are transposons with several mutations within their host organisms. To gain insight into the patterns of the variations, nucleotide substitutions and indels of transposons were analysed in Chilo suppressalis Walker. The CsuPLE1.1 is a member of the piggyBac-like element (PLE) family, which belongs to the DNA transposons, and the Csu-Ty3 is a member of the Ty3/gypsy family, which belongs to the RNA transposons. Copies of CsuPLE1.1 and Csu-Ty3 were cloned separately from different C. suppressalis individuals, and then multiple sequence alignments were performed. There were numerous single-base substitutions in CsuPLE1.1 and Csu-Ty3, but only a few insertion and deletion mutations. Similarly, in both transposons, the occurring frequencies of transitions were significantly higher than transversions (p ≤ 0.01). In the single-base substitutions, the most frequently occurring base changes were A→G and T→C in both types of transposons. Additionally, single-base substitution frequencies occurring at positions 1, 2 or 3 (pos1, pos2 or pos3) of a given codon in the element transposase were not significantly different. Both in CsuPLE1.1 and Csu-Ty3, the patterns of nucleotide substitution had the same characteristics and nucleotide mutations were biased toward GC. This research provides a perspective on the understanding of transposon mutation patterns. View Full-Text
Keywords: transposon; transition; transversion; indels; patterns transposon; transition; transversion; indels; patterns
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MDPI and ACS Style

Luo, G.-H.; Li, X.-H.; Han, Z.-J.; Zhang, Z.-C.; Yang, Q.; Guo, H.-F.; Fang, J.-C. Transition and Transversion Mutations Are Biased towards GC in Transposons of Chilo suppressalis (Lepidoptera: Pyralidae). Genes 2016, 7, 72.

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