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Genes 2015, 6(4), 1268-1282; doi:10.3390/genes6041268

Characterization of Thermotolerant Chitinases Encoded by a Brevibacillus laterosporus Strain Isolated from a Suburban Wetland

College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China
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Academic Editor: Thierry Wirth
Received: 7 October 2015 / Revised: 20 November 2015 / Accepted: 27 November 2015 / Published: 4 December 2015
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Abstract

To isolate and characterize chitinases that can be applied with practical advantages, 57 isolates of chitin-degrading bacteria were isolated from the soil of a suburban wetland. 16S rRNA gene analysis revealed that the majority of these strains belonged to two genera, Paenibacillus and Brevibacillus. Taking thermostability into account, the chitinases (ChiA and ChiC) of a B. laterosporus strain were studied further. Ni-NTA affinity-purified ChiA and ChiC were optimally active at pH 7.0 and 6.0, respectively, and showed high temperature stability up to 55 °C. Kinetic analysis revealed that ChiC has a lower affinity and stronger catalytic activity toward colloidal chitin than ChiA. With their stability in a broad temperature range, ChiA and ChiC can be utilized for the industrial bioconversion of chitin wastes into biologically active products. View Full-Text
Keywords: Brevibacillus laterosporus; chitinase; kinetic analysis; enzymatic properties Brevibacillus laterosporus; chitinase; kinetic analysis; enzymatic properties
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Liu, P.; Cheng, D.; Miao, L. Characterization of Thermotolerant Chitinases Encoded by a Brevibacillus laterosporus Strain Isolated from a Suburban Wetland. Genes 2015, 6, 1268-1282.

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