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Genes 2015, 6(4), 1215-1229; doi:10.3390/genes6041215

Identification of Candidate Genes for Seed Glucosinolate Content Using Association Mapping in Brassica napus L.

1,2,3,†
,
1,2,†
,
1,2
,
1,2
,
1,2
,
1,2
,
1,2
,
1,2
,
1,2
and
1,2,*
1
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Tiansheng Road 2, Beibei, Chongqing 400716, China
2
Engineering Research Center of South Upland Agriculture of Ministry of Education, Southwest University, Beibei, Chongqing 400716, China
3
Food and Bioproduct Science, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editors: Sean Mayes, Festo Massawe, Prakit Somta and Wai Kuan Ho
Received: 22 September 2015 / Revised: 22 October 2015 / Accepted: 6 November 2015 / Published: 18 November 2015
(This article belongs to the Special Issue Genetic Diversity for Crop Improvement)
View Full-Text   |   Download PDF [1216 KB, uploaded 18 November 2015]   |  

Abstract

Rapeseed contains glucosinolates, a toxic group of sulfur-containing glucosides, which play critical roles in defense against herbivores and microbes. However, the presence of glucosinolates in rapeseed reduces the value of the meal as feed for livestock. We performed association mapping of seed glucosinolate (GS) content using the 60K Brassica Infinium single nucleotide polymorphism (SNP) array in 520 oilseed rape accessions. A total of 11 peak SNPs significantly associated with GS content were detected in growing seasons of 2013 and 2014 and were located on B. napus chromosomes A08, A09, C03, and C09, respectively. Two associated regions of GS content covered by these markers were further verified, and three B. napus homologous genes involved in the biosynthesis and accumulation of GS were identified. These genes were multigene family members and were distributed on different chromosomes. Moreover, two genes (BnGRT2 and BnMYB28) associated with GS content were validated by the qRT-PCR analysis of their expression profiles. The further identification and functionalization of these genes will provide useful insight into the mechanism underlying GS biosynthesis and allocation in B. napus, and the associated SNPs markers could be helpful for molecular maker-assisted breeding for low seed GS in B. napus. View Full-Text
Keywords: Brassica napus L.; seed glucosinolate (GS) content; association mapping; single nucleotide polymorphism (SNP) Brassica napus L.; seed glucosinolate (GS) content; association mapping; single nucleotide polymorphism (SNP)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Qu, C.-M.; Li, S.-M.; Duan, X.-J.; Fan, J.-H.; Jia, L.-D.; Zhao, H.-Y.; Lu, K.; Li, J.-N.; Xu, X.-F.; Wang, R. Identification of Candidate Genes for Seed Glucosinolate Content Using Association Mapping in Brassica napus L.. Genes 2015, 6, 1215-1229.

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