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Meiosis in a Bottle: New Approaches to Overcome Mammalian Meiocyte Study Limitations
Genes 2011, 2(1), 260-279; doi:10.3390/genes2010260

NEK1 Facilitates Cohesin Removal during Mammalian Spermatogenesis

1,* , 1
1 Department of Biomedical Sciences, Cornell University, Ithaca, NY 14853, USA 2 Department of Biochemistry, Laboratory for Macromolecular Analysis & Proteomics (LMAP), Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, NY 10461, USA
* Author to whom correspondence should be addressed.
Received: 19 January 2011 / Revised: 18 February 2011 / Accepted: 23 February 2011 / Published: 7 March 2011
(This article belongs to the Special Issue Genetics of Mammalian Meiosis)
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Meiosis is a highly conserved process, which is stringently regulated in all organisms, from fungi through to humans. Two major events define meiosis in eukaryotes. The first is the pairing, or synapsis, of homologous chromosomes and the second is the exchange of genetic information in a process called meiotic recombination. Synapsis is mediated by the meiosis-specific synaptonemal complex structure in combination with the cohesins that tether sister chromatids together along chromosome arms through prophase I. Previously, we identified FKBP6 as a novel component of the mammalian synaptonemal complex. Further studies demonstrated an interaction between FKBP6 and the NIMA-related kinase-1, NEK1. To further investigate the role of NEK1 in mammalian meiosis, we have examined gametogenesis in the spontaneous mutant, Nek1kat2J. Homozygous mutant animals show decreased testis size, defects in testis morphology, and in cohesin removal at late prophase I of meiosis, causing complete male infertility. Cohesin protein SMC3 remains localized to the meiotic chromosome cores at diplonema in the Nek1 mutant, and also in the related Fkbp6 mutant, while in wild type cells SMC3 is removed from the cores at the end of prophase I and becomes more diffuse throughout the DAPI stained region of the nucleus. These data implicate NEK1 as a possible kinase involved in cohesin redistribution in murine spermatocytes.
Keywords: meiosis; cohesin; kinase; fertility; spermatocyte; mouse; NIMA-like meiosis; cohesin; kinase; fertility; spermatocyte; mouse; NIMA-like
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Holloway, K.; Roberson, E.C.; Corbett, K.L.; Kolas, N.K.; Nieves, E.; Cohen, P.E. NEK1 Facilitates Cohesin Removal during Mammalian Spermatogenesis. Genes 2011, 2, 260-279.

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