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Genes 2010, 1(2), 227-243; doi:10.3390/genes1020227
Review

Next Generation Sequencing of Ancient DNA: Requirements, Strategies and Perspectives

1,*  and 2
Received: 31 May 2010; in revised form: 20 July 2010 / Accepted: 23 July 2010 / Published: 28 July 2010
(This article belongs to the Special Issue Next Generation DNA Sequencing)
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Abstract: The invention of next-generation-sequencing has revolutionized almost all fields of genetics, but few have profited from it as much as the field of ancient DNA research. From its beginnings as an interesting but rather marginal discipline, ancient DNA research is now on its way into the centre of evolutionary biology. In less than a year from its invention next-generation-sequencing had increased the amount of DNA sequence data available from extinct organisms by several orders of magnitude. Ancient DNA  research is now not only adding a temporal aspect to evolutionary studies and allowing for the observation of evolution in real time, it also provides important data to help understand the origins of our own species. Here we review progress that has been made in next-generation-sequencing of ancient DNA over the past five years and evaluate sequencing strategies and future directions.
Keywords: ancient DNA; barcoding; genome sequencing; hybridization capture; targeted sequencing ancient DNA; barcoding; genome sequencing; hybridization capture; targeted sequencing
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Knapp, M.; Hofreiter, M. Next Generation Sequencing of Ancient DNA: Requirements, Strategies and Perspectives. Genes 2010, 1, 227-243.

AMA Style

Knapp M, Hofreiter M. Next Generation Sequencing of Ancient DNA: Requirements, Strategies and Perspectives. Genes. 2010; 1(2):227-243.

Chicago/Turabian Style

Knapp, Michael; Hofreiter, Michael. 2010. "Next Generation Sequencing of Ancient DNA: Requirements, Strategies and Perspectives." Genes 1, no. 2: 227-243.


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