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Genes 2010, 1(2), 227-243; doi:10.3390/genes1020227

Next Generation Sequencing of Ancient DNA: Requirements, Strategies and Perspectives

Allan Wilson Centre for Molecular Ecology and Evolution, Department of Anatomy and Structural Biology, University of Otago, 270 Great King Street, 9016 Dunedin, New Zealand
Department of Biology, The University of York, Wentworth Way, Heslington, YO10 5DD, York, UK
Author to whom correspondence should be addressed.
Received: 31 May 2010 / Revised: 20 July 2010 / Accepted: 23 July 2010 / Published: 28 July 2010
(This article belongs to the Special Issue Next Generation DNA Sequencing)
View Full-Text   |   Download PDF [321 KB, uploaded 28 July 2010]   |  


The invention of next-generation-sequencing has revolutionized almost all fields of genetics, but few have profited from it as much as the field of ancient DNA research. From its beginnings as an interesting but rather marginal discipline, ancient DNA research is now on its way into the centre of evolutionary biology. In less than a year from its invention next-generation-sequencing had increased the amount of DNA sequence data available from extinct organisms by several orders of magnitude. Ancient DNA research is now not only adding a temporal aspect to evolutionary studies and allowing for the observation of evolution in real time, it also provides important data to help understand the origins of our own species. Here we review progress that has been made in next-generation-sequencing of ancient DNA over the past five years and evaluate sequencing strategies and future directions. View Full-Text
Keywords: ancient DNA; barcoding; genome sequencing; hybridization capture; targeted sequencing ancient DNA; barcoding; genome sequencing; hybridization capture; targeted sequencing

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Knapp, M.; Hofreiter, M. Next Generation Sequencing of Ancient DNA: Requirements, Strategies and Perspectives. Genes 2010, 1, 227-243.

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