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Cells 2017, 6(4), 37; https://doi.org/10.3390/cells6040037

Standard Assays for the Study of Autophagy in the Ex Vivo Retina

Departament of Cellular and Molecular Biology, Centro de Investigaciones Biológicas, CSIC, 28040 Madrid, Spain
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Received: 20 September 2017 / Revised: 17 October 2017 / Accepted: 17 October 2017 / Published: 22 October 2017
(This article belongs to the Special Issue Assays to Monitor Autophagy in Model Systems)
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Abstract

Autophagy is a catabolic pathway that mediates the degradation and recycling of intracellular components, and is a key player in a variety of physiological processes in cells and tissues. Recent studies of autophagy in the eye suggest that this pathway is fundamental for the preservation of retinal homeostasis. Given its accessible location outside the brain, the retina is an ideal organ in which to study the central nervous system and a wide range of neuronal processes, from development to neurodegeneration. Here we review several methods used to assess autophagy in the retina in both physiological and pathological conditions. View Full-Text
Keywords: autophagy; retina; organotypic cultures; lysosomes; mitophagy autophagy; retina; organotypic cultures; lysosomes; mitophagy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Gómez-Sintes, R.; Villarejo-Zori, B.; Serrano-Puebla, A.; Esteban-Martínez, L.; Sierra-Filardi, E.; Ramírez-Pardo, I.; Rodríguez-Muela, N.; Boya, P. Standard Assays for the Study of Autophagy in the Ex Vivo Retina. Cells 2017, 6, 37.

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