Previous Article in Journal / Special Issue
Exploring Autophagy in Drosophila
Article Menu

Export Article

Open AccessFeature PaperReview
Cells 2017, 6(3), 23; doi:10.3390/cells6030023

Assays to Monitor Autophagy in Saccharomyces cerevisiae

Max F. Perutz Laboratories, University of Vienna, Vienna Biocenter (VBC), Dr.-Bohr-Gasse 9, 1030 Vienna, Austria
Authors to whom correspondence should be addressed.
Received: 8 June 2017 / Revised: 4 July 2017 / Accepted: 9 July 2017 / Published: 13 July 2017
(This article belongs to the Special Issue Assays to Monitor Autophagy in Model Systems)
View Full-Text   |   Download PDF [3896 KB, uploaded 13 July 2017]   |  


Autophagy is an intracellular process responsible for the degradation and recycling of cytoplasmic components. It selectively removes harmful cellular material and enables the cell to survive starvation by mobilizing nutrients via the bulk degradation of cytoplasmic components. While research over the last decades has led to the discovery of the key factors involved in autophagy, the pathway is not yet completely understood. The first studies of autophagy on a molecular level were conducted in the yeast Saccharomyces cerevisiae. Building up on these studies, many homologs have been found in higher eukaryotes. Yeast remains a highly relevant model organism for studying autophagy, with a wide range of established methods to elucidate the molecular details of the autophagy pathway. In this review, we provide an overview of methods to study both selective and bulk autophagy, including intermediate steps in the yeast Saccharomyces cerevisiae. We compare different assays, discuss their advantages and limitations and list potential applications. View Full-Text
Keywords: yeast; bulk autophagy; Cvt pathway; selective autophagy; PAS; autophagosome; autophagic body; iPass; M-Track yeast; bulk autophagy; Cvt pathway; selective autophagy; PAS; autophagosome; autophagic body; iPass; M-Track

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Torggler, R.; Papinski, D.; Kraft, C. Assays to Monitor Autophagy in Saccharomyces cerevisiae. Cells 2017, 6, 23.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top