Abstract: Transient receptor potential (TRP) ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are implicated in modulation of cough and nociception. In vivo, TRPA1 and TRPV1 are often co-expressed in neurons and TRPA1V1 hetero-tetramer formation is noted in cells co-transfected with the respective expression plasmids. In order to understand the impact of TRP receptor interaction on activity, we created stable cell lines expressing the TRPA1, TRPV1 and co-expressing the TRPA1 and TRPV1 (TRPA1V1) receptors. Among the 600 compounds screened against these receptors, we observed a number of compounds that activated the TRPA1, TRPV1 and TRPA1V1 receptors; compounds that activated TRPA1 and TRPA1V1; compounds that activated TRPV1 and TRPA1V1; compounds in which TRPA1V1 response was modulated by either TRPA1 or TRPV1; and compounds that activated only TRPV1 or TRPA1 or TRPA1V1; and one compound that activated TRPA1 and TRPV1, but not TRPA1V1. These results suggest that co-expression of TRPA1 and TRPV1 receptors imparts unique activation profiles different from that of cells expressing only TRPA1 or TRPV1.
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Sadofsky, L.R.; Sreekrishna, K.T.; Lin, Y.; Schinaman, R.; Gorka, K.; Mantri, Y.; Haught, J.C.; Huggins, T.G.; Isfort, R.J.; Bascom, C.C.; Morice, A.H. Unique Responses are Observed in Transient Receptor Potential Ankyrin 1 and Vanilloid 1 (TRPA1 and TRPV1) Co-Expressing Cells. Cells 2014, 3, 616-626.
Sadofsky LR, Sreekrishna KT, Lin Y, Schinaman R, Gorka K, Mantri Y, Haught JC, Huggins TG, Isfort RJ, Bascom CC, Morice AH. Unique Responses are Observed in Transient Receptor Potential Ankyrin 1 and Vanilloid 1 (TRPA1 and TRPV1) Co-Expressing Cells. Cells. 2014; 3(2):616-626.
Sadofsky, Laura R.; Sreekrishna, Koti T.; Lin, Yakang; Schinaman, Renee; Gorka, Kate; Mantri, Yogita; Haught, John C.; Huggins, Thomas G.; Isfort, Robert J.; Bascom, Charles C.; Morice, Alyn H. 2014. "Unique Responses are Observed in Transient Receptor Potential Ankyrin 1 and Vanilloid 1 (TRPA1 and TRPV1) Co-Expressing Cells." Cells 3, no. 2: 616-626.