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Cells 2014, 3(2), 616-626; doi:10.3390/cells3020616
Article

Unique Responses are Observed in Transient Receptor Potential Ankyrin 1 and Vanilloid 1 (TRPA1 and TRPV1) Co-Expressing Cells

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1 Cardiovascular and Respiratory Studies, The University of Hull, HU6 7RX, UK 2 The Procter & Gamble Company, Mason, OH 45040, USA
* Author to whom correspondence should be addressed.
Received: 4 April 2014 / Revised: 29 May 2014 / Accepted: 30 May 2014 / Published: 11 June 2014
(This article belongs to the Special Issue Transient Receptor Potential (TRP) Channels)
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Abstract

Transient receptor potential (TRP) ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) receptors are implicated in modulation of cough and nociception. In vivo, TRPA1 and TRPV1 are often co-expressed in neurons and TRPA1V1 hetero-tetramer formation is noted in cells co-transfected with the respective expression plasmids. In order to understand the impact of TRP receptor interaction on activity, we created stable cell lines expressing the TRPA1, TRPV1 and co-expressing the TRPA1 and TRPV1 (TRPA1V1) receptors. Among the 600 compounds screened against these receptors, we observed a number of compounds that activated the TRPA1, TRPV1 and TRPA1V1 receptors; compounds that activated TRPA1 and TRPA1V1; compounds that activated TRPV1 and TRPA1V1; compounds in which TRPA1V1 response was modulated by either TRPA1 or TRPV1; and compounds that activated only TRPV1 or TRPA1 or TRPA1V1; and one compound that activated TRPA1 and TRPV1, but not TRPA1V1. These results suggest that co-expression of TRPA1 and TRPV1 receptors imparts unique activation profiles different from that of cells expressing only TRPA1 or TRPV1.
Keywords: TRPV1; TRPA1; TRPA1; TRPV1 co-expression TRPV1; TRPA1; TRPA1; TRPV1 co-expression
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Sadofsky, L.R.; Sreekrishna, K.T.; Lin, Y.; Schinaman, R.; Gorka, K.; Mantri, Y.; Haught, J.C.; Huggins, T.G.; Isfort, R.J.; Bascom, C.C.; Morice, A.H. Unique Responses are Observed in Transient Receptor Potential Ankyrin 1 and Vanilloid 1 (TRPA1 and TRPV1) Co-Expressing Cells. Cells 2014, 3, 616-626.

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