Next Article in Journal
An Enhanced ELISPOT Assay for Sensitive Detection of Antigen-Specific T Cell Responses to Borrelia burgdorferi
Next Article in Special Issue
Actin in Action: Imaging Approaches to Study Cytoskeleton Structure and Function
Previous Article in Journal
Interorganellar Membrane Microdomains: Dynamic Platforms in the Control of Calcium Signaling and Apoptosis
Previous Article in Special Issue
A Drosophila Model to Image Phagosome Maturation
Cells 2013, 2(3), 591-606; doi:10.3390/cells2030591
Review

Fluorescein Derivatives in Intravital Fluorescence Imaging

1,* , 2 and 1,3
Received: 14 June 2013; in revised form: 25 July 2013 / Accepted: 26 July 2013 / Published: 2 August 2013
(This article belongs to the Special Issue Imaging in Cell Biology and Development)
View Full-Text   |   Download PDF [2012 KB, uploaded 2 August 2013]   |   Browse Figures
Abstract: Intravital fluorescence microscopy enables the direct imaging of fluorophores in vivo and advanced techniques such as fluorescence lifetime imaging (FLIM) enable the simultaneous detection of multiple fluorophores. Consequently, it is now possible to record distribution and metabolism of a chemical in vivo and to optimise the delivery of fluorophores in vivo. Recent clinical applications with fluorescein and other intravital fluorescent stains have occurred in neurosurgery, dermatology [including photodynamic therapy (PDT)] and endomicroscopy. Potential uses have been identified in periodontal disease, skin graft and cancer surgery. Animal studies have demonstrated that diseased tissue can be specifically stained with fluorophore conjugates. This review focuses on the fluorescein derived fluorophores in common clinical use and provides examples of novel applications from studies in tissue samples.
Keywords: intravital; FLIM; fluorescein intravital; FLIM; fluorescein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Robertson, T.A.; Bunel, F.; Roberts, M.S. Fluorescein Derivatives in Intravital Fluorescence Imaging. Cells 2013, 2, 591-606.

AMA Style

Robertson TA, Bunel F, Roberts MS. Fluorescein Derivatives in Intravital Fluorescence Imaging. Cells. 2013; 2(3):591-606.

Chicago/Turabian Style

Robertson, Thomas A.; Bunel, Florestan; Roberts, Michael S. 2013. "Fluorescein Derivatives in Intravital Fluorescence Imaging." Cells 2, no. 3: 591-606.


Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert