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Cells 2013, 2(1), 105-123; doi:10.3390/cells2010105
Review

Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways

 and
*
The Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Victoria 3010, Australia
* Author to whom correspondence should be addressed.
Received: 19 December 2012 / Revised: 5 February 2013 / Accepted: 7 February 2013 / Published: 22 February 2013
(This article belongs to the Special Issue Imaging in Cell Biology and Development)
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Abstract

Recent improvements in the resolution of light microscopy, coupled with the development of a range of fluorescent-based probes, have provided new approaches to dissecting membrane domains and the regulation of membrane trafficking. Here, we review these advances, as well as highlight developments in quantitative image analysis and novel unbiased analytical approaches to quantitate protein localization. The application of these approaches to endosomal sorting and endosome-to-Golgi transport is discussed.
Keywords: optical imaging; super-resolution microscopy; flow cytometry; Golgi apparatus; endosomes, fluorescent proteins optical imaging; super-resolution microscopy; flow cytometry; Golgi apparatus; endosomes, fluorescent proteins
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chia, P.Z.C.; Gleeson, P.A. Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways. Cells 2013, 2, 105-123.

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