Cells 2013, 2(1), 105-123; doi:10.3390/cells2010105
Review

Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways

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Received: 19 December 2012; in revised form: 5 February 2013 / Accepted: 7 February 2013 / Published: 22 February 2013
(This article belongs to the Special Issue Imaging in Cell Biology and Development)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Recent improvements in the resolution of light microscopy, coupled with the development of a range of fluorescent-based probes, have provided new approaches to dissecting membrane domains and the regulation of membrane trafficking. Here, we review these advances, as well as highlight developments in quantitative image analysis and novel unbiased analytical approaches to quantitate protein localization. The application of these approaches to endosomal sorting and endosome-to-Golgi transport is discussed.
Keywords: optical imaging; super-resolution microscopy; flow cytometry; Golgi apparatus; endosomes, fluorescent proteins
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MDPI and ACS Style

Chia, P.Z.C.; Gleeson, P.A. Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways. Cells 2013, 2, 105-123.

AMA Style

Chia PZC, Gleeson PA. Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways. Cells. 2013; 2(1):105-123.

Chicago/Turabian Style

Chia, Pei Z.C.; Gleeson, Paul A. 2013. "Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways." Cells 2, no. 1: 105-123.

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