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Cells 2013, 2(1), 105-123; doi:10.3390/cells2010105
Review
Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways
The Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Victoria 3010, Australia
* Author to whom correspondence should be addressed.
Received: 19 December 2012; in revised form: 5 February 2013 / Accepted: 7 February 2013 / Published: 22 February 2013
(This article belongs to the Special Issue Imaging in Cell Biology and Development)
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Abstract: Recent improvements in the resolution of light microscopy, coupled with the development of a range of fluorescent-based probes, have provided new approaches to dissecting membrane domains and the regulation of membrane trafficking. Here, we review these advances, as well as highlight developments in quantitative image analysis and novel unbiased analytical approaches to quantitate protein localization. The application of these approaches to endosomal sorting and endosome-to-Golgi transport is discussed.
Keywords: optical imaging; super-resolution microscopy; flow cytometry; Golgi apparatus; endosomes, fluorescent proteins
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MDPI and ACS Style
Chia, P.Z.C.; Gleeson, P.A. Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways. Cells 2013, 2, 105-123.
AMA StyleChia PZC, Gleeson PA. Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways. Cells. 2013; 2(1):105-123.
Chicago/Turabian StyleChia, Pei Z.C.; Gleeson, Paul A. 2013. "Imaging and Quantitation Techniques for Tracking Cargo along Endosome-to-Golgi Transport Pathways." Cells 2, no. 1: 105-123.