Marker Assisted Transfer of Stripe Rust and Stem Rust Resistance Genes into Four Wheat Cultivars
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Pathogen Materials
2.3. Greenhouse Tests
2.4. Field Tests
2.5. DNA Isolation and Quantification
2.6. PCR Amplification and Gel Electrophoresis
2.7. Marker Assisted Selection
2.8. Chi-Squared Analyses
3. Results
3.1. Marker Assisted Transfer
3.2. Yr51
3.3. Yr57
3.4. Sr22
3.5. Sr26
3.6. Sr50
3.7. Marker Assisted Selection of Gene Combinations Among BCF2 Families
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Recurrent Parents | Donor Parents | Target Gene | Backcross Progenies |
---|---|---|---|
Gladius, Livingston, PBW550, DBW17 | AUS91456 | Yr51 | BC2F2 |
AUS91463 | Yr57 | BC2F2 | |
Sr22/3*K441 | Sr22 | BC1F2 | |
Sr26 WA1 | Sr26 | BC1F2 | |
Dra-1/Chinese Spring ph1b/2/3* Gabo | Sr50 | BC1F2 |
Gene | Chromosome | Marker | Dominant/Co-Dominant | Base Pairs/Allele | Source | |
---|---|---|---|---|---|---|
Donor Parent | Recurrent Parent | |||||
Lr34 | 7DS | csLV34 | Co-dominant | 150 | 229 | Lagudah et al. [19] |
Lr37 | 2AS | VENTRIUP-LN2 | Dominant | 252 | Null | Helguera et al. [20] |
Yr51 | 4AL | sun104 | Dominant | 225 | Null | Randhawa et al. [11] |
Yr57 | 3BS | gwm389 | Co-dominant | 150 | 145 or 147 | Randhawa et al. [12] |
Yr57 | 3BS | BS00062676 | Co-dominant | A:A | G:G | Randhawa et al. [12] |
Sr2 | 3BS | csSr2 | Co-dominant | 172, 112, 53 | 225,112 | Mago et al. [21] |
Sr22 | 7AL | cssu22 | Co-dominant | 237 | 335 | Periyannan et al. [13] |
Sr24 | 3DL | Sr24#12 | Dominant | 500 | Null | Mago et al. [14] |
Sr26 | 6AL | Sr26#43 | Dominant | 270 | Null | Mago et al. [14] |
Sr50 | 1DL.1RS | Sr50-5p-F3, R2 | Dominant | 470 | Null | Mago et al. [15] |
Genotype | Pedigree | Infection type | |
---|---|---|---|
134 E 16A+Yr17+Yr27+ | 34-1,2,3,4,5,6,7 | ||
Donor parent | |||
AUS91456 | AUS27858/Westonia RIL #5515-1 | ;n-1n | - |
AUS91463 | AUS27858/Westonia RIL #5474-6 | 0; | - |
Sr22/3*K441 | - | - | ;1+ |
Sr26 WA1 | - | - | ;1+c |
Dra-1/Chinese Spring ph1b/2/3* Gabo | - | - | ;1++c |
Recurrent parent | |||
Gladius | (DH)Rac-875/Krichauff//Excalibur /Kukri/3/RAC-875/Krichauff/4/RAC-875//Excalibur/Kukri | 3+ | ;1 |
Livingston | SUN-129-A/Sunvale | 3+ | 23c |
PBW550 | WH-594/RAJ-3856//W-485 | 3+ | ;1− |
DBW17 | CMH-79-A-95/3*Ciano79//RAJ-3777 | 3+ | ;1 |
BC1F1 | BC2F1 | |||||||
Cross | Yr51yr51 | yr51yr51 | χ2 (1:1) | p-value | Yr51yr51 | yr51yr51 | χ2 (1:1) | p-value |
Yr51/Gladius | 11 | 8 | 0.47 | 0.49 | 22 | 14 | 1.78 | 0.18 |
Yr51/Livingston | 9 | 8 | 0.06 | 0.81 | 14 | 11 | 0.36 | 0.55 |
Yr51/PBW550 | 11 | 7 | 0.89 | 0.35 | 9 | 4 | 1.92 | 0.16 |
Yr51/DBW17 | 11 | 9 | 0.20 | 0.65 | 6 | 14 | 3.20 | 0.07 |
Yr57yr57 | yr57yr57 | Yr57yr57 | yr57yr57 | |||||
Yr57/Gladius | 10 | 10 | 0.00 | 1.00 | 11 | 11 | 0.00 | 1.00 |
Yr57/Livingston | 14 | 6 | 3.20 | 0.07 | 14 | 6 | 3.20 | 0.07 |
Yr57/PBW550 | 6 | 5 | 0.09 | 0.76 | 8 | 9 | 0.06 | 0.80 |
Yr57/DBW17 | 10 | 9 | 0.05 | 0.82 | 7 | 4 | 0.82 | 0.37 |
BC1F1 | BC1F2 | |||||||
Sr22sr22 | sr22sr22 | Sr22sr22 | sr22sr22 | χ2 (3:1) | ||||
Sr22/Gladius | 4 | 6 | 0.40 | 0.53 | 9 | 7 | 3.00 | 0.08 |
Sr22/Livingston | 5 | 4 | 0.11 | 0.74 | 13 | 3 | 0.33 | 0.56 |
Sr22/PBW550 | 3 | 5 | 0.50 | 0.48 | 10 | 6 | 1.33 | 0.25 |
Sr22/DBW17 | 7 | 5 | 0.33 | 0.56 | 13 | 3 | 0.33 | 0.56 |
Sr26sr26 | Sr26sr26 | Sr26sr26 | sr26sr26 | |||||
Sr26/Gladius | 5 | 5 | 0.00 | 1.00 | 13 | 3 | 0.33 | 0.56 |
Sr26/Livingston | 5 | 5 | 0.00 | 1.00 | 10 | 6 | 1.33 | 0.25 |
Sr26/PBW550 | 4 | 5 | 0.11 | 0.74 | 14 | 2 | 1.33 | 0.25 |
Sr26/DBW17 | 3 | 9 | 3.00 | 0.08 | 12 | 4 | 0.00 | 1.00 |
Sr50sr50 | sr50sr50 | Sr50sr50 | sr50sr50 | |||||
Sr50/Gladius | 7 | 3 | 1.60 | 0.20 | 9 | 7 | 3.00 | 0.08 |
Sr50/Livingston | 4 | 6 | 0.40 | 0.53 | 10 | 6 | 1.33 | 0.25 |
Sr50/PBW550 | 4 | 6 | 0.40 | 0.53 | 11 | 5 | 0.33 | 0.56 |
Sr50/DBW17 | 6 | 6 | 0.00 | 1.00 | 15 | 1 | 3.00 | 0.08 |
Recurrent Parents | Background Gene (s) | Gene Combinations | Number of Selected Plants |
---|---|---|---|
Gladius | Lr37/Yr17/Sr38, Lr24/Sr24 | Yr51 + Lr37/Yr17/Sr38+ Lr24/Sr24 | 4 |
Yr57 + Lr37/Yr17/Sr38 + Lr24/Sr24 | 7 | ||
Sr22 + Lr37/Yr17/Sr38 + Lr24/Sr24 | 7 | ||
Sr26 + Lr37/Yr17/Sr38 + Lr24/Sr24 | 13 | ||
Sr50 + Lr37/Yr17/Sr38 + Lr24/Sr24 | 8 | ||
Livingston | Lr34/Yr18/Sr57, Lr37/Yr17/Sr38, Sr2 | Yr51 + Lr34/Yr18/Sr57 + Lr37/Yr17/Sr38 | 4 |
Yr57 + Lr34/Yr18/Sr57 + Lr37/Yr17/Sr38 | 11 | ||
Sr22 + Lr34/Yr18/Sr57+ Lr37/Yr17/Sr38 | 12 | ||
Sr26 + Lr34/Yr18/Sr57 + Lr37/Yr17/Sr38 | 2 | ||
Sr50 + Lr34/Yr18/Sr57 + Lr37/Yr17/Sr38 | 4 | ||
PBW550 | Lr34/Yr18/Sr57 | Yr51 + Lr34/Yr18/Sr57 | 3 |
Yr57 + Lr34/Yr18/Sr57 | 3 | ||
Sr22 + Lr34/Yr18/Sr57 | 10 | ||
Sr26 + Lr34/Yr18/Sr57 | 4 | ||
Sr50 + Lr34/Yr18/Sr57 | 4 | ||
DBW17 | Lr34/Yr18/Sr57 | Yr51 + Lr34/Yr18/Sr57 | 3 |
Yr57 + Lr34/Yr18/Sr57 | 2 | ||
Sr22 + Lr34/Yr18/Sr57 | 9 | ||
Sr26 + Lr34/Yr18/Sr57 | 3 | ||
Sr50 + Lr34/Yr18/Sr57 | 5 |
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Randhawa, M.S.; Bains, N.S.; Sohu, V.S.; Chhuneja, P.; Trethowan, R.M.; Bariana, H.S.; Bansal, U. Marker Assisted Transfer of Stripe Rust and Stem Rust Resistance Genes into Four Wheat Cultivars. Agronomy 2019, 9, 497. https://doi.org/10.3390/agronomy9090497
Randhawa MS, Bains NS, Sohu VS, Chhuneja P, Trethowan RM, Bariana HS, Bansal U. Marker Assisted Transfer of Stripe Rust and Stem Rust Resistance Genes into Four Wheat Cultivars. Agronomy. 2019; 9(9):497. https://doi.org/10.3390/agronomy9090497
Chicago/Turabian StyleRandhawa, Mandeep S., Navtej S. Bains, Virinder S. Sohu, Parveen Chhuneja, Richard M. Trethowan, Harbans S. Bariana, and Urmil Bansal. 2019. "Marker Assisted Transfer of Stripe Rust and Stem Rust Resistance Genes into Four Wheat Cultivars" Agronomy 9, no. 9: 497. https://doi.org/10.3390/agronomy9090497