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Agronomy 2017, 7(3), 50; doi:10.3390/agronomy7030050

Detection and Response of Sugarcane against the Infection of Sugarcane Mosaic Virus (SCMV) in Indonesia

1
Division of Biology Molecule and Biotechnology, Center for Development of Advanced Sciences and Technology, The University of Jember, Jalan Kalimantan 37 Kampus Tegalboto, Jemb©er, East Java 68121, Indonesia
2
Faculty of Agriculture, The University of Jember, Jember, East Java 68121, Indonesia
3
Division of Research and Quality Control, PT. Perkebunan Nusantara XI. Jl. Merak No.1, Krembangan Selatan, Krembangan, Kota Surabaya, East Java 60175, Indonesia
4
Department of Biology, Faculty of Science and Technology, Airlangga University, Jl. Airlangga No. 4–6, Gubeng, Kota Surabaya, East Java 60115, Indonesia
5
Faculty of Math and Sciences, The University of Jember, Jember, East Java 68121, Indonesia
*
Author to whom correspondence should be addressed.
Academic Editor: Urmila Basu
Received: 16 June 2017 / Revised: 5 July 2017 / Accepted: 10 July 2017 / Published: 24 July 2017
View Full-Text   |   Download PDF [1784 KB, uploaded 24 July 2017]   |  

Abstract

Sugarcane mosaic virus (SCMV) is one among many viruses that infect sugarcane, cause yield loss, and become serious disease agents on sugarcane plantations. Since the morphological symptoms of SCMV are similar to other symptoms caused by Sugarcane streak mosaic virus (SCSMV) or nitrogen deficiency, the detection of SCMV is important through accurate diagnostic-like ELISA or RT-PCR. This research aimed to study the causative mosaic pathogen of SCMV in East Java, Indonesia, including mosaic development. The results showed that the mosaic symptom is present in all sugarcane plantations with 78% and 65% disease incidence and severity, respectively. Moreover, the detection procedure based on an amplification of cDNA of the coat protein gene sequence confirmed that SCMV was the causative agent of mosaic disease on sugarcane. Re-inoculation of healthy sugarcane plants with plant sap from a symptomatic leaf from the field showed similar mosaic or yellowish chlorotic areas on the leaf blade, and appeared on the fourth leaves upward from the inoculation leaf, in addition to showing different levels of peroxidase but not total phenol. Mosaic also correlated with the amount of total chlorophyll. Although Sucrose phosphate synthase (SPS) protein accumulation and activity were at a lower level in infected leaves, sucrose accumulation was at a higher level in the same leaves. View Full-Text
Keywords: Sugarcane mosaic virus; sugarcane; RT-PCR; plant response Sugarcane mosaic virus; sugarcane; RT-PCR; plant response
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MDPI and ACS Style

Addy, H.S.; Nurmalasari; Wahyudi, A.H.S.; Sholeh, A.; Anugrah, C.; Iriyanto, F.E.S.; Darmanto, W.; Sugiharto, B. Detection and Response of Sugarcane against the Infection of Sugarcane Mosaic Virus (SCMV) in Indonesia. Agronomy 2017, 7, 50.

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