Next Article in Journal
Impact of Different Agricultural Waste Biochars on Maize Biomass and Soil Water Content in a Brazilian Cerrado Arenosol
Previous Article in Journal
Ultrasound Assisted Extraction for the Recovery of Phenolic Compounds from Vegetable Sources
Previous Article in Special Issue
Applying New Technologies to Transform Blueberry Harvesting
Article Menu

Export Article

Open AccessArticle
Agronomy 2017, 7(3), 48; doi:10.3390/agronomy7030048

Effect of Constitutive miR164 Expression on Plant Morphology and Fruit Development in Arabidopsis and Tomato

1
Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional (CIBA-IPN), Ex-Hacienda San Juan Molino, Carretera Estatal Tecuexcomac-Tepetitla Km 1.5, Tlaxcala, CP 90700 Tepetitla, Mexico
2
Unidad de Genómica Avanzada (LANGEBIO), Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Km. 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, CP 36821 Guanajuato, Mexico
3
Laboratorio de Transformación, Departamento de Ingeniería Genética, Unidad Irapuato, CINVESTAV-IPN, Km. 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, CP 36821 Guanajuato, Mexico
4
Departamento de Biotecnología y Bioquímica, Unidad Irapuato, CINVESTAV-IPN, Km. 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, CP 36821 Guanajuato, Mexico
These authors contributed to this work equally.
*
Authors to whom correspondence should be addressed.
Academic Editors: Esther van der Knaap and Cecilia E. McGregor
Received: 13 April 2017 / Revised: 7 July 2017 / Accepted: 11 July 2017 / Published: 15 July 2017
View Full-Text   |   Download PDF [2049 KB, uploaded 15 July 2017]   |  

Abstract

Several miRNAs are conserved in different plant families, but their abundance and target genes vary between species, organs, and stages of development. Target genes of miRNAs are mostly transcription factors, involved in the control of many plant developmental processes, including fruit development. MiR164 is a conserved miRNA, highly expressed in fruits, and is validated to target a subset of genes of the NAC-domain transcription factor gene family. The objective of this work was to analyze the phenotypic effects of the constitutive expression of miR164 during the life cycle of Arabidopsis and tomato. MiR164 overexpression (164-OE) lines for Arabidopsis and tomato were generated and analyzed during plant development. The constitutive miR164 expression showed that miR164 affected the morphology of Arabidopsis and tomato, and it affected the transition from the vegetative to the reproductive phase in Arabidopsis. Moreover, the miR164 overexpression affected the time required for each developmental stage of tomato fruit. These results suggest that miR164 plays general and specific roles during development in Arabidopsis and tomato, including fruit development, which could be exploited for the improvement of traits of agronomic interest in important species. View Full-Text
Keywords: miRNA; miR164; overexpression miRNA; fruit development; tomato; Arabidopsis miRNA; miR164; overexpression miRNA; fruit development; tomato; Arabidopsis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Rosas Cárdenas, F.F.; Ruiz Suárez, Y.; Cano Rangel, R.M.; Luna Garcia, V.; González Aguilera, K.L.; Marsch Martínez, N.; de Folter, S. Effect of Constitutive miR164 Expression on Plant Morphology and Fruit Development in Arabidopsis and Tomato. Agronomy 2017, 7, 48.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Agronomy EISSN 2073-4395 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top