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Agronomy 2015, 5(1), 71-88; doi:10.3390/agronomy5010071

A Combined Field/Laboratory Method for Assessment of Frost Tolerance with Freezing Tests and Chlorophyll Fluorescence

CRA-GPG—Council for Agricultural Research and Economics, Genomics Research Centre, Via San Protaso 302, Fiorenzuola d'Arda (PC) 29017, Italy
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Academic Editor: Tristan Coram
Received: 4 December 2014 / Revised: 12 February 2015 / Accepted: 3 March 2015 / Published: 17 March 2015
(This article belongs to the Special Issue Field Phenotyping for Yield and Environmental Stress Tolerance Traits)
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Abstract

Recent progress in genotyping allows for studies of the molecular genetic basis of cold resistance in cereals. However, as in many other fields of molecular genetic analysis, phenotyping for high numbers of genotypes is still a major bottleneck. The use of chlorophyll fluorescence measurements as an indicator for freezing stress is a well established and rapid method for evaluation of frost tolerance. In order to extend the applicability of this technique beyond plants grown under controlled conditions in growth chambers and sacrificed for the test, here we study its applicability for leaves harvested from field trials during winter and subjected to freezing tests. Such an approach allows for simultaneous studies of the advancement of cold hardening and other components of winter survival apart from frost tolerance. It is shown that cutting or senescence of cut leaves does not have adverse effects on the outcome of subsequent freezing stress tests. The time requirements for field sampling and laboratory testing on high numbers of genotypes allow for the application of the proposed approach for genotyping/phenotyping studies. View Full-Text
Keywords: phenotyping; genotyping; frost tolerance; hardening; freezing test; chlorophyll fluorescence; field-laboratory method; cereals phenotyping; genotyping; frost tolerance; hardening; freezing test; chlorophyll fluorescence; field-laboratory method; cereals
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Badeck, F.-W.; Rizza, F. A Combined Field/Laboratory Method for Assessment of Frost Tolerance with Freezing Tests and Chlorophyll Fluorescence. Agronomy 2015, 5, 71-88.

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