Agronomy 2013, 3(1), 117-134; doi:10.3390/agronomy3010117
Article

Genetic Variation in Soybean at the Maturity Locus E4 Is Involved in Adaptation to Long Days at High Latitudes

1,†email, 2,†email, 3,†email, 4,* email, 5email, 5email, 4email, 4email, 6email, 1email, 2,7email, 1email and 3,* email
Received: 11 December 2012; in revised form: 10 January 2013 / Accepted: 25 January 2013 / Published: 4 February 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Soybean (Glycine max) cultivars adapted to high latitudes have a weakened or absent sensitivity to photoperiod. The purposes of this study were to determine the molecular basis for photoperiod insensitivity in various soybean accessions, focusing on the sequence diversity of the E4 (GmphyA2) gene, which encodes a phytochrome A (phyA) protein, and its homoeolog (GmphyA1), and to disclose the evolutionary consequences of two phyA homoeologs after gene duplication. We detected four new single-base deletions in the exons of E4, all of which result in prematurely truncated proteins. A survey of 191 cultivated accessions sourced from various regions of East Asia with allele-specific molecular markers reliably determined that the accessions with dysfunctional alleles were limited to small geographical regions, suggesting the alleles’ recent and independent origins from functional E4 alleles. Comparison of nucleotide diversity values revealed lower nucleotide diversity at non-synonymous sites in GmphyA1 than in E4, although both have accumulated mutations at almost the same rate in synonymous and non-coding regions. Natural mutations have repeatedly generated loss-of-function alleles at the E4 locus, and these have accumulated in local populations. The E4 locus is a key player in the adaptation of soybean to high-latitude environments under diverse cropping systems.
Keywords: genetic diversity; soybean; flowering; phytochrome A; photoperiod insensitivity
PDF Full-text Download PDF Full-Text [384 KB, uploaded 4 February 2013 08:55 CET]

Export to BibTeX |
EndNote


MDPI and ACS Style

Tsubokura, Y.; Matsumura, H.; Xu, M.; Liu, B.; Nakashima, H.; Anai, T.; Kong, F.; Yuan, X.; Kanamori, H.; Katayose, Y.; Takahashi, R.; Harada, K.; Abe, J. Genetic Variation in Soybean at the Maturity Locus E4 Is Involved in Adaptation to Long Days at High Latitudes. Agronomy 2013, 3, 117-134.

AMA Style

Tsubokura Y, Matsumura H, Xu M, Liu B, Nakashima H, Anai T, Kong F, Yuan X, Kanamori H, Katayose Y, Takahashi R, Harada K, Abe J. Genetic Variation in Soybean at the Maturity Locus E4 Is Involved in Adaptation to Long Days at High Latitudes. Agronomy. 2013; 3(1):117-134.

Chicago/Turabian Style

Tsubokura, Yasutaka; Matsumura, Hisakazu; Xu, Meilan; Liu, Baohui; Nakashima, Hiroko; Anai, Toyoaki; Kong, Fanjiang; Yuan, Xiaohui; Kanamori, Hiroyuki; Katayose, Yuichi; Takahashi, Ryoji; Harada, Kyuya; Abe, Jun. 2013. "Genetic Variation in Soybean at the Maturity Locus E4 Is Involved in Adaptation to Long Days at High Latitudes." Agronomy 3, no. 1: 117-134.

Agronomy EISSN 2073-4395 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert