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Polymers 2017, 9(9), 450; https://doi.org/10.3390/polym9090450

Osteogenesis of Adipose-Derived and Bone Marrow Stem Cells with Polycaprolactone/Tricalcium Phosphate and Three-Dimensional Printing Technology in a Dog Model of Maxillary Bone Defects

1
Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University, Daegu 41944, Korea
2
Cell & Matrix Research Institute, School of Medicine, Kyungpook National University, Daegu 41944, Korea
3
Department of Mechanical Engineering, Korea Polytechnic University, Siheung-Si 15073, Korea
4
Department of Prosthodontics, Dental Research Institute, Institute of Translational Dental Science, School of Dentistry, Pusan National University, Yangsan-Si 50612, Korea
5
Department of Medicine, School of Medicine, Konkuk University, Seoul 05029, Korea
6
College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea
*
Author to whom correspondence should be addressed.
Received: 25 July 2017 / Revised: 12 September 2017 / Accepted: 12 September 2017 / Published: 15 September 2017
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Abstract

Bone graft material should possess sufficient porosity and permeability to allow integration with native tissue and vascular invasion, and must satisfy oxygen and nutrient transport demands. In this study, we have examined the use of three-dimensional (3D)-printed polycaprolactone/tricalcium phosphate (PCL/TCP) composite material in bone grafting, to estimate the scope of its potential application in bone surgery. Adipose-derived stem cells (ADSCs) and bone marrow stem cells (BMSCs) are known to enhance osteointegration. We hypothesized that a patient-specific 3D-printed solid scaffold could help preserve seeded ADSCs and BMSCs and enhance osteointegration. Diffuse osteogenic tissue formation was observed by micro-computed tomography with both stem cell types, and the ADSC group displayed similar osteogenesis compared to the BMSC group. In histological assessment, the scaffold pores showed abundant ossification in both groups. Reverse transcription polymerase chain reaction (RT-PCR) showed that the BMSC group had higher expression of genes associated with ossification, and this was confirmed by Western blot analysis. The ADSC- and BMSC-seeded 3D-printed PCL/TCP scaffolds displayed promising enhancement of osteogenesis in a dog model of maxillary bone defects. View Full-Text
Keywords: adipose-derived stem cells; bone marrow stem cells; osteogenesis; 3D-printed scaffold; polycaprolactone; tricalcium phosphate; dog; maxillary bone adipose-derived stem cells; bone marrow stem cells; osteogenesis; 3D-printed scaffold; polycaprolactone; tricalcium phosphate; dog; maxillary bone
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Lee, J.W.; Chu, S.G.; Kim, H.T.; Choi, K.Y.; Oh, E.J.; Shim, J.-H.; Yun, W.-S.; Huh, J.B.; Moon, S.H.; Kang, S.S.; Chung, H.Y. Osteogenesis of Adipose-Derived and Bone Marrow Stem Cells with Polycaprolactone/Tricalcium Phosphate and Three-Dimensional Printing Technology in a Dog Model of Maxillary Bone Defects. Polymers 2017, 9, 450.

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