Fabrication of Multi-Layered Lidocaine and Epinephrine-Eluting PLGA/Collagen Nanofibers: In Vitro and In Vivo Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. In Vitro Release of Epinephrine and Lidocaine
2.1.1. Manufacture of Polycaprolactone Stent and Drugs Loaded PLGA Nanofibers
2.1.2. Characterization of Electrospun Nanofibrous Membranes
2.1.3. Drug Concentration Assessment
2.2. Cytotoxicity of Composite Nanofibers
2.3. In Vivo Study of Animal
2.3.1. Animal-Related Procedure
2.3.2. Post-Surgery Evaluation
2.3.3. Wound Healing and Histological Analysis
2.4. Statistical Analysis
3. Results
3.1. In Vitro Characterization of Electrospun Drug-Loaded Nanofibrous Membranes
3.2. In Vitro Elution Characteristics of Epinephrine and Lidocaine
3.3. In Vivo Elution of Lidocaine and Epinephrine
3.4. Efficacy of Released Drugs
3.4.1. Hemostasis Efficacy
3.4.2. Weight Changes
3.4.3. Food Intake and Water Consumption
3.4.4. Wound Healing and Histological Analysis
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Lee, F.-Y.; Lee, D.; Lee, T.-C.; Chen, J.-K.; Wu, R.-C.; Liu, K.-C.; Liu, S.-J. Fabrication of Multi-Layered Lidocaine and Epinephrine-Eluting PLGA/Collagen Nanofibers: In Vitro and In Vivo Study. Polymers 2017, 9, 416. https://doi.org/10.3390/polym9090416
Lee F-Y, Lee D, Lee T-C, Chen J-K, Wu R-C, Liu K-C, Liu S-J. Fabrication of Multi-Layered Lidocaine and Epinephrine-Eluting PLGA/Collagen Nanofibers: In Vitro and In Vivo Study. Polymers. 2017; 9(9):416. https://doi.org/10.3390/polym9090416
Chicago/Turabian StyleLee, Fu-Ying, Demei Lee, Tzu-Chia Lee, Jan-Kan Chen, Ren-Chin Wu, Kuan-Chieh Liu, and Shih-Jung Liu. 2017. "Fabrication of Multi-Layered Lidocaine and Epinephrine-Eluting PLGA/Collagen Nanofibers: In Vitro and In Vivo Study" Polymers 9, no. 9: 416. https://doi.org/10.3390/polym9090416