Next Article in Journal
Microencapsulation of Lactobacillus Acidophilus by Xanthan-Chitosan and Its Stability in Yoghurt
Next Article in Special Issue
Effect of Cationic Surface Modification on the Rheological Behavior and Microstructure of Nanocrystalline Cellulose
Previous Article in Journal
Preparation and Evaluation of a Polyimide-Coated Ultrafine Gilt Molybdenum Wire and Its Knitted Mesh Used for Electromagnetic Reflectors
Previous Article in Special Issue
The Effects of Using Sodium Alginate Hydrosols Treated with Direct Electric Current as Coatings for Sausages
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Polymers 2017, 9(12), 736; https://doi.org/10.3390/polym9120736

Immobilization of Platelet-Rich Plasma onto COOH Plasma-Coated PCL Nanofibers Boost Viability and Proliferation of Human Mesenchymal Stem Cells

1
Scientific Institute of Clinical and Experimental Lymphology-Branch of the ICG SB RAS, 2 Timakova str., 630060 Novosibirsk, Russia
2
National University of Science and Technology “MISiS”, Leninsky pr. 4, 119049 Moscow, Russia
3
Research Institute of Biochemistry, 2 Timakova str., 630117 Novosibirsk, Russia
4
RG Plasma Technologies, CEITEC–Central European Institute of Technology, Masaryk University, Purkyňova 123, 61200 Brno, Czech Republic
5
Chair of Physical Metallurgy and Materials Technology, Brandenburg Technical University, 03046 Cottbus, Germany
6
CEITEC-Central European Institute of Technology, Brno University of Technology, Technická 3058/10, 61600 Brno, Czech Republic
7
Institute of Physical Engineering, Brno University of Technology, Technicka 2896/2, 616 69 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Received: 9 November 2017 / Revised: 6 December 2017 / Accepted: 18 December 2017 / Published: 20 December 2017
(This article belongs to the Special Issue Surface Modification and Functional Coatings for Polymers)
View Full-Text   |   Download PDF [6700 KB, uploaded 22 December 2017]   |  

Abstract

The scaffolds made of polycaprolactone (PCL) are actively employed in different areas of biology and medicine, especially in tissue engineering. However, the usage of unmodified PCL is significantly restricted by the hydrophobicity of its surface, due to the fact that its inert surface hinders the adhesion of cells and the cell interactions on PCL surface. In this work, the surface of PCL nanofibers is modified by Ar/CO2/C2H4 plasma depositing active COOH groups in the amount of 0.57 at % that were later used for the immobilization of platelet-rich plasma (PRP). The modification of PCL nanofibers significantly enhances the viability and proliferation (by hundred times) of human mesenchymal stem cells, and decreases apoptotic cell death to a normal level. According to X-ray photoelectron spectroscopy (XPS), after immobilization of PRP, up to 10.7 at % of nitrogen was incorporated into the nanofibers surface confirming the grafting of proteins. Active proliferation and sustaining the cell viability on nanofibers with immobilized PRP led to an average number of cells of 258 ± 12.9 and 364 ± 34.5 for nanofibers with ionic and covalent bonding of PRP, respectively. Hence, our new method for the modification of PCL nanofibers with PRP opens new possibilities for its application in tissue engineering. View Full-Text
Keywords: polycaprolactone; nanofibers; COOH plasma; platelet-rich plasma; cell viability; PRP immobilization polycaprolactone; nanofibers; COOH plasma; platelet-rich plasma; cell viability; PRP immobilization
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Solovieva, A.; Miroshnichenko, S.; Kovalskii, A.; Permyakova, E.; Popov, Z.; Dvořáková, E.; Kiryukhantsev-Korneev, P.; Obrosov, A.; Polčak, J.; Zajíčková, L.; Shtansky, D.V.; Manakhov, A. Immobilization of Platelet-Rich Plasma onto COOH Plasma-Coated PCL Nanofibers Boost Viability and Proliferation of Human Mesenchymal Stem Cells. Polymers 2017, 9, 736.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Polymers EISSN 2073-4360 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top