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Polymers 2016, 8(2), 51; doi:10.3390/polym8020051

Conformation and Rheological Properties of Calf-Thymus DNA in Solution

1
Grenoble Alpes University, Laboratoire Rhéologie et Procédés (LRP), F-38000, Grenoble 38000, France
2
Biomaterials applications, 6 rue Lesdiguières, Grenoble 38000, France
3
Departamento de Ingeniería Química, Universidad de Guadalajara, Blvd. M. García Barragán, Guadalajara C.P. 44430, Jalisco, Mexico
*
Author to whom correspondence should be addressed.
Received: 7 January 2016 / Revised: 3 February 2016 / Accepted: 5 February 2016 / Published: 11 February 2016
(This article belongs to the Collection Polyelectrolytes)
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Abstract

Studies of DNA molecule behavior in aqueous solutions performed through different approaches allow assessment of the solute-solvent interactions and examination of the strong influence of conformation on its physicochemical properties, in the presence of different ionic species and ionic concentrations. Firstly, the conformational behavior of calf-thymus DNA molecules in TE buffer solution is presented as a function of temperature. Secondly, their rheological behavior is discussed, as well as the evidence of the critical concentrations, i.e., the overlap and the entanglement concentrations (C* and Ce, respectively) from steady state flow and oscillatory dynamic shear experiments. The determination of the viscosity in the Newtonian plateau obtained from flow curves η ( ) allows estimation of the intrinsic viscosity and the specific viscosities at zero shear when C[η] < 40. At end, a generalized master curve is obtained from the variation of the specific viscosity as a function of the overlap parameter C[η]. The variation of the exponent s obtained from the power law η~ s for both flow and dynamic results is discussed in terms of Graessley’s analysis. In the semi-dilute regime with entanglements, a dynamic master curve is obtained as a function of DNA concentration (CDNA > 2.0 mg/mL) and temperature (10 °C < T < 40 °C). View Full-Text
Keywords: DNA; hydrodynamic behavior; conformation; electrostatic interactions; temperature DNA; hydrodynamic behavior; conformation; electrostatic interactions; temperature
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Bravo-Anaya, L.M.; Rinaudo, M.; Martínez, F.A.S. Conformation and Rheological Properties of Calf-Thymus DNA in Solution. Polymers 2016, 8, 51.

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