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Polymers 2014, 6(4), 1164-1177; doi:10.3390/polym6041164

On the Interaction of Adherent Cells with Thermoresponsive Polymer Coatings

1,* , 2,†
1 Fraunhofer Institute for Biomedical Engineering (IBMT), Potsdam 14476, Germany 2 Max Planck Institute for Colloids and Interfaces, Potsdam 14476, Germany 3 Fraunhofer Institute for Applied Polymer Research (IAP), Potsdam 14476, Germany Present affiliation: Humboldt University, Berlin 12489, Germany Present affiliation: Institute Charles Sadron, Strasbourg 67000, France
* Author to whom correspondence should be addressed.
Received: 2 March 2014 / Revised: 1 April 2014 / Accepted: 4 April 2014 / Published: 15 April 2014
(This article belongs to the Special Issue Polymer Colloids)
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Thermoresponsive polymer coatings allow the control of adhesion of cells on synthetic substrates. In particular, decreasing the temperature below the lower critical solution temperature (LCST) of the polymer triggers the non-invasive detachment of cells from their cultivation substrate. Widening the range of applications of these coatings in cellular biotechnology requires a better understanding of their interaction with cells. By monitoring the morphological changes of cells during their detachment at various temperatures, we provide evidence that cell detachment is an active process. Analyses of cell residues that are left behind by the cells on the substrate during their detachment, further support this notion. In the second part of this work, we show that the kinetics of adhesion and the efficiency of detachment of cells can be controlled through the coadsorption of molecules bearing the peptide motif RGD (arginine-glycine-aspartic acid) with the polymers.
Keywords: thermoresponsive polymer; RGD; cell detachment; polyethylene glycol thermoresponsive polymer; RGD; cell detachment; polyethylene glycol
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Uhlig, K.; Boerner, H.G.; Wischerhoff, E.; Lutz, J.-F.; Jaeger, M.S.; Laschewsky, A.; Duschl, C. On the Interaction of Adherent Cells with Thermoresponsive Polymer Coatings. Polymers 2014, 6, 1164-1177.

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