Polymers 2014, 6(4), 1164-1177; doi:10.3390/polym6041164

On the Interaction of Adherent Cells with Thermoresponsive Polymer Coatings

1,* email, 2,†email, 3email, 3,‡email, 1email, 3email and 1email
Received: 2 March 2014; in revised form: 1 April 2014 / Accepted: 4 April 2014 / Published: 15 April 2014
(This article belongs to the Special Issue Polymer Colloids)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Thermoresponsive polymer coatings allow the control of adhesion of cells on synthetic substrates. In particular, decreasing the temperature below the lower critical solution temperature (LCST) of the polymer triggers the non-invasive detachment of cells from their cultivation substrate. Widening the range of applications of these coatings in cellular biotechnology requires a better understanding of their interaction with cells. By monitoring the morphological changes of cells during their detachment at various temperatures, we provide evidence that cell detachment is an active process. Analyses of cell residues that are left behind by the cells on the substrate during their detachment, further support this notion. In the second part of this work, we show that the kinetics of adhesion and the efficiency of detachment of cells can be controlled through the coadsorption of molecules bearing the peptide motif RGD (arginine-glycine-aspartic acid) with the polymers.
Keywords: thermoresponsive polymer; RGD; cell detachment; polyethylene glycol
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MDPI and ACS Style

Uhlig, K.; Boerner, H.G.; Wischerhoff, E.; Lutz, J.-F.; Jaeger, M.S.; Laschewsky, A.; Duschl, C. On the Interaction of Adherent Cells with Thermoresponsive Polymer Coatings. Polymers 2014, 6, 1164-1177.

AMA Style

Uhlig K, Boerner HG, Wischerhoff E, Lutz J-F, Jaeger MS, Laschewsky A, Duschl C. On the Interaction of Adherent Cells with Thermoresponsive Polymer Coatings. Polymers. 2014; 6(4):1164-1177.

Chicago/Turabian Style

Uhlig, Katja; Boerner, Hans G.; Wischerhoff, Erik; Lutz, Jean-François; Jaeger, Magnus S.; Laschewsky, André; Duschl, Claus. 2014. "On the Interaction of Adherent Cells with Thermoresponsive Polymer Coatings." Polymers 6, no. 4: 1164-1177.

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