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Polymers 2011, 3(1), 571-586; doi:10.3390/polym3010571
Article

PLGA-Based Microparticles for the Sustained Release of BMP-2

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Received: 9 December 2010; in revised form: 18 January 2011 / Accepted: 24 February 2011 / Published: 1 March 2011
(This article belongs to the Special Issue Biofunctional Polymers for Medical Applications)
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Abstract: The development of growth factor delivery strategies to circumvent the burst release phenomenon prevalent in most current systems has driven research towards encapsulating molecules in resorbable polymer matrices. For these polymer release techniques to be efficacious in a clinical setting, several key points need to be addressed. This present study has investigated the encapsulation of the growth factor, BMP-2 within PLGA/PLGA-PEG-PLGA microparticles. Morphology, size distribution, encapsulation efficiency and release kinetics were investigated and we have demonstrated a sustained release of bioactive BMP-2. Furthermore, biocompatibility of the PLGA microparticles was established and released BMP-2 was shown to promote the differentiation of MC3T3-E1 cells towards the osteogenic lineage to a greater extent than osteogenic supplements (as early as day 10 in culture), as determined using alkaline phosphatase and alizarin red assays. This study showcases a potential BMP-2 delivery system which may now be translated into more complex delivery systems, such as 3D, mechanically robust scaffolds for bone tissue regeneration applications.
Keywords: poly(lactic-co-glycolic acid); PLGA; poly(ethylene glycol) PEG; MC3T3; differentiation; BMP-2; microparticles; microspheres; controlled release poly(lactic-co-glycolic acid); PLGA; poly(ethylene glycol) PEG; MC3T3; differentiation; BMP-2; microparticles; microspheres; controlled release
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kirby, G.T.S.; White, L.J.; Rahman, C.V.; Cox, H.C.; Qutachi, O.; Rose, F.R.A.J.; Hutmacher, D.W.; Shakesheff, K.M.; Woodruff, M.A. PLGA-Based Microparticles for the Sustained Release of BMP-2. Polymers 2011, 3, 571-586.

AMA Style

Kirby GTS, White LJ, Rahman CV, Cox HC, Qutachi O, Rose FRAJ, Hutmacher DW, Shakesheff KM, Woodruff MA. PLGA-Based Microparticles for the Sustained Release of BMP-2. Polymers. 2011; 3(1):571-586.

Chicago/Turabian Style

Kirby, Giles T. S.; White, Lisa J.; Rahman, Cheryl V.; Cox, Helen C.; Qutachi, Omar; Rose, Felicity R. A. J.; Hutmacher, Dietmar W.; Shakesheff, Kevin M.; Woodruff, Maria A. 2011. "PLGA-Based Microparticles for the Sustained Release of BMP-2." Polymers 3, no. 1: 571-586.


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