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PLGA-Based Microparticles for the Sustained Release of BMP-2
School of Pharmacy, University of Nottingham, University Park, Nottingham, UK
Regenerative Medicine Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia
Biomaterials and Tissue Morphology Group, Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove, Queensland, Australia
* Author to whom correspondence should be addressed.
Received: 9 December 2010; in revised form: 18 January 2011 / Accepted: 24 February 2011 / Published: 1 March 2011
Abstract: The development of growth factor delivery strategies to circumvent the burst release phenomenon prevalent in most current systems has driven research towards encapsulating molecules in resorbable polymer matrices. For these polymer release techniques to be efficacious in a clinical setting, several key points need to be addressed. This present study has investigated the encapsulation of the growth factor, BMP-2 within PLGA/PLGA-PEG-PLGA microparticles. Morphology, size distribution, encapsulation efficiency and release kinetics were investigated and we have demonstrated a sustained release of bioactive BMP-2. Furthermore, biocompatibility of the PLGA microparticles was established and released BMP-2 was shown to promote the differentiation of MC3T3-E1 cells towards the osteogenic lineage to a greater extent than osteogenic supplements (as early as day 10 in culture), as determined using alkaline phosphatase and alizarin red assays. This study showcases a potential BMP-2 delivery system which may now be translated into more complex delivery systems, such as 3D, mechanically robust scaffolds for bone tissue regeneration applications.
Keywords: poly(lactic-co-glycolic acid); PLGA; poly(ethylene glycol) PEG; MC3T3; differentiation; BMP-2; microparticles; microspheres; controlled release
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Kirby, G.T.S.; White, L.J.; Rahman, C.V.; Cox, H.C.; Qutachi, O.; Rose, F.R.A.J.; Hutmacher, D.W.; Shakesheff, K.M.; Woodruff, M.A. PLGA-Based Microparticles for the Sustained Release of BMP-2. Polymers 2011, 3, 571-586.
Kirby GTS, White LJ, Rahman CV, Cox HC, Qutachi O, Rose FRAJ, Hutmacher DW, Shakesheff KM, Woodruff MA. PLGA-Based Microparticles for the Sustained Release of BMP-2. Polymers. 2011; 3(1):571-586.
Kirby, Giles T. S.; White, Lisa J.; Rahman, Cheryl V.; Cox, Helen C.; Qutachi, Omar; Rose, Felicity R. A. J.; Hutmacher, Dietmar W.; Shakesheff, Kevin M.; Woodruff, Maria A. 2011. "PLGA-Based Microparticles for the Sustained Release of BMP-2." Polymers 3, no. 1: 571-586.