A 3D Electroactive Polypyrrole-Collagen Fibrous Scaffold for Tissue Engineering
AbstractFibers that can provide topographical, biochemical and electrical cues would be attractive for directing the differentiation of stem cells into electro-responsive cells such as neuronal or muscular cells. Here we report on the fabrication of polypyrrole-incorporated collagen-based fibers via interfacial polyelectrolyte complexation (IPC). The mean ultimate tensile strength of the fibers is 304.0 ± 61.0 MPa and the Young’s Modulus is 10.4 ± 4.3 GPa. Human bone marrow-derived mesenchymal stem cells (hMSCs) are cultured on the fibers in a proliferating medium and stimulated with an external electrical pulse generator for 5 and 10 days. The effects of polypyrrole in the fiber system can be observed, with hMSCs adopting a neuronal-like morphology at day 10, and through the upregulation of neural markers, such as noggin, MAP2, neurofilament, β tubulin III and nestin. This study demonstrates the potential of this fiber system as an attractive 3D scaffold for tissue engineering, where collagen is present on the fiber surface for cellular adhesion, and polypyrrole is encapsulated within the fiber for enhanced electrical communication in cell-substrate and cell-cell interactions.
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Yow, S.-Z.; Lim, T.H.; Yim, E.K.F.; Lim, C.T.; Leong, K.W. A 3D Electroactive Polypyrrole-Collagen Fibrous Scaffold for Tissue Engineering. Polymers 2011, 3, 527-544.
Yow S-Z, Lim TH, Yim EKF, Lim CT, Leong KW. A 3D Electroactive Polypyrrole-Collagen Fibrous Scaffold for Tissue Engineering. Polymers. 2011; 3(1):527-544.Chicago/Turabian Style
Yow, Soh-Zeom; Lim, Tze Han; Yim, Evelyn K. F.; Lim, Chwee Teck; Leong, Kam W. 2011. "A 3D Electroactive Polypyrrole-Collagen Fibrous Scaffold for Tissue Engineering." Polymers 3, no. 1: 527-544.