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Polymers 2018, 10(1), 54; https://doi.org/10.3390/polym10010054

Facile and Rapid Formation of Giant Vesicles from Glass Beads

1
Department of Chemistry, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland
2
Department of Biochemistry, University of Geneva, 30, Quai Ernest-Ansermet, 1211 Geneva, Switzerland
3
National Centre of Competence in Research in Chemical Biology, 1211 Geneva, Switzerland
*
Author to whom correspondence should be addressed.
Received: 8 December 2017 / Revised: 31 December 2017 / Accepted: 4 January 2018 / Published: 9 January 2018
(This article belongs to the Special Issue Selected Papers from "ECIS 2017")
View Full-Text   |   Download PDF [2957 KB, uploaded 9 January 2018]   |  

Abstract

Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesicles of uniform size using standard laboratory equipment is, therefore, highly desirable. Here, the gentle hydration method of glass bead-supported thin lipid films has been enhanced by adding a vortexing step. This led to the formulation of a uniform population of giant vesicles. Batches of glass beads coated with different lipids can be combined to produce vesicles of hybrid lipid compositions. This method represents a stable approach to rapidly generate giant vesicles. View Full-Text
Keywords: formulation techniques; glass bead technique; giant vesicles; hybrid vesicles; phospholipids formulation techniques; glass bead technique; giant vesicles; hybrid vesicles; phospholipids
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Tanasescu, R.; Mettal, U.; Colom, A.; Roux, A.; Zumbuehl, A. Facile and Rapid Formation of Giant Vesicles from Glass Beads. Polymers 2018, 10, 54.

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