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Crystals 2016, 6(8), 95; doi:10.3390/cryst6080095

Technique for High-Quality Protein Crystal Growth by Control of Subgrain Formation under an External Electric Field

1
Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan
2
Graduate School of Nanobioscience, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan
3
Department of Education, Yokohama Soei University, 1 Miho-tyou, Midori-ku, Yokohama 226-0015, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Helmut Cölfen and Ronald W. Armstrong
Received: 30 March 2016 / Revised: 4 August 2016 / Accepted: 6 August 2016 / Published: 16 August 2016
(This article belongs to the Special Issue Crystal Dislocations)
View Full-Text   |   Download PDF [1008 KB, uploaded 16 August 2016]   |  

Abstract

X-ray diffraction (XRD) rocking-curves were measured for tetragonal hen egg white (HEW) lysozyme crystals grown with and without application of an external electric field, and the crystal quality was assessed according to the full width at half-maximums (FWHMs) of each rocking-curve profile. The average FWHMs for tetragonal HEW lysozyme crystals grown with an external electric field at 1 MHz were smaller than those for crystals grown without, especially for the 12 12 0 reflection. The crystal homogeneity of the tetragonal HEW lysozyme crystals was also improved under application of an external electric field at 1 MHz, compared to that without. Improvement of the crystal quality of tetragonal HEW lysozyme crystals grown under an applied field is discussed with a focus on subgrain formation. In addition, the origin of subgrain misorientation is also discussed with respect to the incorporation of impurities into protein crystals. View Full-Text
Keywords: protein crystals; crystal quality; electric field; rocking curve protein crystals; crystal quality; electric field; rocking curve
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Koizumi, H.; Uda, S.; Fujiwara, K.; Tachibana, M.; Kojima, K.; Nozawa, J. Technique for High-Quality Protein Crystal Growth by Control of Subgrain Formation under an External Electric Field. Crystals 2016, 6, 95.

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