Production of 4-Ene-3-ketosteroids in Corynebacterium glutamicum
AbstractCorynebacterium glutamicum has been widely used for the industrial production of amino acids and many value-added chemicals; however, it has not been exploited for the production of steroids. Using C. glutamicum as a cellular biocatalyst we have expressed the 3β-hydroxysteroid dehydrogenase/isomerase MSMEG_5228 from Mycobacterium smegmatis to demonstrate that the resulting recombinant strain is able to oxidize in vivo C19 and C21 3-OH-steroids to their corresponding keto-derivatives. This new approach constitutes a proof of concept of a biotechnological process for producing value-added intermediates such as 4-pregnen-16α,17α-epoxy-16β-methyl-3,20-dione. View Full-Text
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García-Fernández, J.; Galán, B.; Felpeto-Santero, C.; Barredo, J.L.; García, J.L. Production of 4-Ene-3-ketosteroids in Corynebacterium glutamicum. Catalysts 2017, 7, 316.
García-Fernández J, Galán B, Felpeto-Santero C, Barredo JL, García JL. Production of 4-Ene-3-ketosteroids in Corynebacterium glutamicum. Catalysts. 2017; 7(11):316.Chicago/Turabian Style
García-Fernández, Julia; Galán, Beatriz; Felpeto-Santero, Carmen; Barredo, José L.; García, José L. 2017. "Production of 4-Ene-3-ketosteroids in Corynebacterium glutamicum." Catalysts 7, no. 11: 316.