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Catalysts 2017, 7(11), 316; doi:10.3390/catal7110316

Production of 4-Ene-3-ketosteroids in Corynebacterium glutamicum

1
Department of Environmental Biotechnology, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain
2
Department of Biotechnology, Crystal Pharma, A Division of Albany Molecular Research Inc. (AMRI), 47151 Boecillo Valladolid, Spain
*
Author to whom correspondence should be addressed.
Received: 29 September 2017 / Revised: 19 October 2017 / Accepted: 20 October 2017 / Published: 27 October 2017
(This article belongs to the Special Issue Biocatalysis and Biotransformations)
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Abstract

Corynebacterium glutamicum has been widely used for the industrial production of amino acids and many value-added chemicals; however, it has not been exploited for the production of steroids. Using C. glutamicum as a cellular biocatalyst we have expressed the 3β-hydroxysteroid dehydrogenase/isomerase MSMEG_5228 from Mycobacterium smegmatis to demonstrate that the resulting recombinant strain is able to oxidize in vivo C19 and C21 3-OH-steroids to their corresponding keto-derivatives. This new approach constitutes a proof of concept of a biotechnological process for producing value-added intermediates such as 4-pregnen-16α,17α-epoxy-16β-methyl-3,20-dione. View Full-Text
Keywords: 3-OH-steroids; biotransformation; dehydrogenase; Rhodococcus and Corynebacterium expression systems 3-OH-steroids; biotransformation; dehydrogenase; Rhodococcus and Corynebacterium expression systems
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MDPI and ACS Style

García-Fernández, J.; Galán, B.; Felpeto-Santero, C.; Barredo, J.L.; García, J.L. Production of 4-Ene-3-ketosteroids in Corynebacterium glutamicum. Catalysts 2017, 7, 316.

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