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Catalysts 2017, 7(10), 302; doi:10.3390/catal7100302

Improved Catalytic Performance of Lipase Supported on Clay/Chitosan Composite Beads

1
College of Chemistry and Chemical Engineering, Shaoxing University, Huancheng West Road 508, Shaoxing 312000, China
2
College of Life Science, Shaoxing University, Huancheng West Road 508, Shaoxing 312000, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Manuel Ferrer
Received: 11 August 2017 / Revised: 29 September 2017 / Accepted: 11 October 2017 / Published: 13 October 2017
(This article belongs to the Special Issue Biocatalysis and Biotransformations)
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Abstract

Clay/chitosan composite beads were prepared and used as the carrier to support lipase by adsorption, to improve the activity and stability of lipase in the hydrolysis of olive oil. Under conditions of pH 6.0, 25 °C and adsorption for 10 h, immobilized lipases on chitosan bead (CB–lipase) and three clay/chitosan composite beads, at different clay to chitosan proportions of 1:8 (CCB-8-lipase), 1:5 (CCB-5-lipase) and 1:3 (CCB-3-lipase), were prepared. By comparing the activity of these immobilized lipases, CCB-5-lipase showed the highest activity, followed by CCB-8-lipase > CCB-3-lipase > CB-lipase; this improvement was attributed to the synergetic effect of enrichment of olive oil by clay at the reaction surface and better biocompatibility of chitosan with lipase molecules. The optimum pH and temperature in the reaction respectively changed from 7.0 and 30 °C for free lipase to 7.5 and 35 °C for immobilized forms. Furthermore, the thermal stability and repeated usability of these immobilized lipases were sequenced as CCB-3-lipase > CCB-5-lipase > CCB-8-lipase > CB–lipase, due to greater rigidity of immobilized lipase with the addition of clay, which was further confirmed by SEM. The study shows that the incorporation of clay with chitosan creates a good synergetic effect to improve the catalytic performance of immobilized lipase on clay/chitosan composite. View Full-Text
Keywords: lipase; immobilization; clay/chitosan composite; hydrolysis; catalytic performance lipase; immobilization; clay/chitosan composite; hydrolysis; catalytic performance
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MDPI and ACS Style

Tu, N.; Shou, J.; Dong, H.; Huang, J.; Li, Y. Improved Catalytic Performance of Lipase Supported on Clay/Chitosan Composite Beads. Catalysts 2017, 7, 302.

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