Preparation of Cross-Linked Glucoamylase Aggregates Immobilization by Using Dextrin and Xanthan Gum as Protecting Agents
Abstract
:1. Introduction
2. Results and Discussion
2.1. Glutaraldehyde Concentration
2.2. Protecting Agents Concentration
2.3. Dependence of Catalytic Activity on pH and Temperature
2.4. Thermostability of Immobilized Glucoamylase
2.5. Kinetic Constants
2.6. Recyclability and Storability
3. Experimental Section
3.1. Preparation
3.1.1. Glucoamylase
3.1.2. Glucoamylase Cross-Linked Enzyme Aggregates (CLEA)
3.2. Determination and Analysis
3.2.1. Activity Assay
3.2.2. Optimum Temperature, pH, and Kinetic Parameters Assay
3.2.3. Determination of Stability
3.2.4. Recoverability
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Types | 60 °C | 70 °C | ||
---|---|---|---|---|
ki (min−1) | t1/2 (min) | ki (min−1) | t1/2 (min) | |
CLEA-XG | 1.49 × 10−4 | 463 ± 15 | 1.67 × 10−3 | 415 ± 18 |
CLEA-D | 9.86 × 10−4 | 703 ± 21 | 2.61 × 10−3 | 266 ± 14 |
free | 4.33 × 10−3 | 160 ± 16 | 1.65 × 10−2 | 42 ± 9 |
Types | Km (μg·L−1) | Vm (μg·min−1·mL−1) | Effectiveness Factor η |
---|---|---|---|
Free | 46 ± 3 | 3.3 × 103 | - |
CLEA-D | 55 ± 5 | 3.1 × 103 | 0.94 |
CLEA-XG | 70 ± 3 | 3.3 × 103 | 0.99 |
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Li, X.-D.; Wu, J.; Jia, D.-C.; Wan, Y.-H.; Yang, N.; Qiao, M. Preparation of Cross-Linked Glucoamylase Aggregates Immobilization by Using Dextrin and Xanthan Gum as Protecting Agents. Catalysts 2016, 6, 77. https://doi.org/10.3390/catal6060077
Li X-D, Wu J, Jia D-C, Wan Y-H, Yang N, Qiao M. Preparation of Cross-Linked Glucoamylase Aggregates Immobilization by Using Dextrin and Xanthan Gum as Protecting Agents. Catalysts. 2016; 6(6):77. https://doi.org/10.3390/catal6060077
Chicago/Turabian StyleLi, Xiao-Dong, Jia Wu, Dong-Chen Jia, Yong-Hu Wan, Na Yang, and Min Qiao. 2016. "Preparation of Cross-Linked Glucoamylase Aggregates Immobilization by Using Dextrin and Xanthan Gum as Protecting Agents" Catalysts 6, no. 6: 77. https://doi.org/10.3390/catal6060077