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Catalysts 2016, 6(6), 77; doi:10.3390/catal6060077

Preparation of Cross-Linked Glucoamylase Aggregates Immobilization by Using Dextrin and Xanthan Gum as Protecting Agents

1
Laboratory for Conservation and Utilization of Bio-resources, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University, Kunming 650091, China
2
College of Pharmacological Sciences, Zhejiang University, Hangzhou 310058, China
*
Author to whom correspondence should be addressed.
Academic Editor: David D. Boehr
Received: 24 March 2016 / Revised: 17 May 2016 / Accepted: 20 May 2016 / Published: 25 May 2016
(This article belongs to the Special Issue Enzyme Catalysis)
View Full-Text   |   Download PDF [1750 KB, uploaded 25 May 2016]   |  

Abstract

In this paper glucoamylase from Aspergillus niger was immobilized by using a modified version of cross-linked enzyme aggregates (CLEA). The co-aggregates were cross-linked with glutaraldehyde; meanwhile dextrin and xanthan gum as protecting agents were added, which provides high affinity with the enzyme molecules. The immobilized glucoamylase was stable over a broad range of pH (3.0–8.0) and temperature (55–75 °C); dependence shows more catalytic activity than a free enzyme. The thermostability, kinetic behavior, and first-order inactivation rate constant (ki) were investigated. The two types of protector made the immobilized glucoamylase more robust than the free form. Both of the immobilized enzymes have excellent recyclability, retaining over 45% of the relative activity after 24 runs. In addition, immobilized enzymes reduced only 40% of the initial activity after three months by the storability measure, indicating high activity. View Full-Text
Keywords: glucoamylase; cross-linked enzyme aggregates (CLEA); immobilized enzyme; protecting agents; substrate glucoamylase; cross-linked enzyme aggregates (CLEA); immobilized enzyme; protecting agents; substrate
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, X.-D.; Wu, J.; Jia, D.-C.; Wan, Y.-H.; Yang, N.; Qiao, M. Preparation of Cross-Linked Glucoamylase Aggregates Immobilization by Using Dextrin and Xanthan Gum as Protecting Agents. Catalysts 2016, 6, 77.

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