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Catalysts 2016, 6(6), 81; doi:10.3390/catal6060081

Role of Conformational Motions in Enzyme Function: Selected Methodologies and Case Studies

1
INRS—Institut Armand-Frappier, Université du Québec, 531 Boul. des Prairies, Laval, QC H7V 1B7, Canada
2
PROTEO, the Québec Network for Research on Protein Function, Engineering, and Applications, 1045 Avenue de la Médecine, Université Laval, Québec, QC G1V 0A6, Canada
3
GRASP, the Groupe de Recherche Axé sur la Structure des Protéines, 3649 Promenade Sir William Osler, McGill University, Montréal, QC H3G 0B1, Canada
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: David D. Boehr
Received: 5 April 2016 / Revised: 11 May 2016 / Accepted: 20 May 2016 / Published: 27 May 2016
(This article belongs to the Special Issue Enzyme Catalysis)
View Full-Text   |   Download PDF [5290 KB, uploaded 27 May 2016]   |  

Abstract

It is now common knowledge that enzymes are mobile entities relying on complex atomic-scale dynamics and coordinated conformational events for proper ligand recognition and catalysis. However, the exact role of protein dynamics in enzyme function remains either poorly understood or difficult to interpret. This mini-review intends to reconcile biophysical observations and biological significance by first describing a number of common experimental and computational methodologies employed to characterize atomic-scale residue motions on various timescales in enzymes, and second by illustrating how the knowledge of these motions can be used to describe the functional behavior of enzymes and even act upon it. Two biologically relevant examples will be highlighted, namely the HIV-1 protease and DNA polymerase β enzyme systems. View Full-Text
Keywords: enzyme catalysis; conformational dynamics; nuclear magnetic resonance; molecular dynamics simulations; HIV-1 protease; DNA polymerase β enzyme catalysis; conformational dynamics; nuclear magnetic resonance; molecular dynamics simulations; HIV-1 protease; DNA polymerase β
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Narayanan, C.; Bernard, D.N.; Doucet, N. Role of Conformational Motions in Enzyme Function: Selected Methodologies and Case Studies. Catalysts 2016, 6, 81.

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