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Catalysts 2016, 6(12), 209; doi:10.3390/catal6120209

Investigation of the Effect of Plasma Polymerized Siloxane Coating for Enzyme Immobilization and Microfluidic Device Conception

1
Institut d’Electronique, de Microélectronique et de Nanotechnologie (IEMN)—UMR CNRS 8520, Université Lille Nord de France—Sciences et Technologies, Villeneuve 59650, d’Ascq, France
2
EA 7394-ICV—Institut Charles Viollette, University of Lille, F-59000 Lille, France
*
Author to whom correspondence should be addressed.
Academic Editor: David D. Boehr
Received: 14 October 2016 / Revised: 25 November 2016 / Accepted: 9 December 2016 / Published: 16 December 2016
(This article belongs to the Special Issue Immobilized Enzymes: Strategies for Enzyme Stabilization)
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Abstract

This paper describes the impact of a physical immobilization methodology, using plasma polymerized 1,1,3,3, tetramethyldisiloxane, on the catalytic performance of β-galactosidase from Aspergillus oryzae in a microfluidic device. The β-galactosidase was immobilized by a polymer coating grown by Plasma Enhanced Chemical Vapor Deposition (PEVCD). Combined with a microchannel patterned in the silicone, a microreactor was obtained with which the diffusion through the plasma polymerized layer and the hydrolysis of a synthetic substrate, the resorufin-β-d-galactopyranoside, were studied. A study of the efficiency of the immobilization procedure was investigated after several uses and kinetic parameters of immobilized β-galactosidase were calculated and compared with those of soluble enzyme. Simulation and a modelling approach were also initiated to understand phenomena that influenced enzyme behavior in the physical immobilization method. Thus, the catalytic performances of immobilized enzymes were directly influenced by immobilization conditions and particularly by the diffusion behavior and availability of substrate molecules in the enzyme microenvironment. View Full-Text
Keywords: physical immobilization; microreactor; plasma polymer; modelling; diffusion physical immobilization; microreactor; plasma polymer; modelling; diffusion
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Belhacene, K.; Elagli, A.; Vivien, C.; Treizebré, A.; Dhulster, P.; Supiot, P.; Froidevaux, R. Investigation of the Effect of Plasma Polymerized Siloxane Coating for Enzyme Immobilization and Microfluidic Device Conception. Catalysts 2016, 6, 209.

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