Next Article in Journal
New Tailor-Made Alkyl-Aldehyde Bifunctional Supports for Lipase Immobilization
Next Article in Special Issue
Investigation of the Effect of Plasma Polymerized Siloxane Coating for Enzyme Immobilization and Microfluidic Device Conception
Previous Article in Journal
Mechanistic and Structural Insight to an Evolved Benzoylformate Decarboxylase with Enhanced Pyruvate Decarboxylase Activity
Previous Article in Special Issue
Adsorption and Activity of Lipase on Polyphosphazene-Modified Polypropylene Membrane Surface
Article Menu

Export Article

Open AccessArticle
Catalysts 2016, 6(12), 189; doi:10.3390/catal6120189

Continuous Packed Bed Reactor with Immobilized β-Galactosidase for Production of Galactooligosaccharides (GOS)

Applied Biocatalysis Group, Instituto de Catálisis y Petroleoquímica, CSIC, 28049 Madrid, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: David D. Boehr
Received: 24 October 2016 / Revised: 23 November 2016 / Accepted: 27 November 2016 / Published: 30 November 2016
(This article belongs to the Special Issue Immobilized Enzymes: Strategies for Enzyme Stabilization)
View Full-Text   |   Download PDF [2832 KB, uploaded 30 November 2016]   |  

Abstract

The β-galactosidase from Bacillus circulans was covalently attached to aldehyde-activated (glyoxal) agarose beads and assayed for the continuous production of galactooligosaccharides (GOS) in a packed-bed reactor (PBR). The immobilization was fast (1 h) and the activity of the resulting biocatalyst was 97.4 U/g measured with o-nitrophenyl-β-d-galactopyranoside (ONPG). The biocatalyst showed excellent operational stability in 14 successive 20 min reaction cycles at 45 °C in a batch reactor. A continuous process for GOS synthesis was operated for 213 h at 0.2 mL/min and 45 °C using 100 g/L of lactose as a feed solution. The efficiency of the PBR slightly decreased with time; however, the maximum GOS concentration (24.2 g/L) was obtained after 48 h of operation, which corresponded to 48.6% lactose conversion and thus to maximum transgalactosylation activity. HPAEC-PAD analysis showed that the two major GOS were the trisaccharide Gal-β(1→4)-Gal-β(1→4)-Glc and the tetrasaccharide Gal-β(1→4)-Gal-β(1→4)-Gal-β(1→4)-Glc. The PBR was also assessed in the production of GOS from milk as a feed solution. The stability of the bioreactor was satisfactory during the first 8 h of operation; after that, a decrease in the flow rate was observed, probably due to partial clogging of the column. This work represents a step forward in the continuous production of GOS employing fixed-bed reactors with immobilized β-galactosidases. View Full-Text
Keywords: galacto-oligosaccharides; prebiotics; transglycosylation; betα-galactosidase; covalent immobilization; Bacillus circulans; glyoxal agarose; fixed-bed reactor galacto-oligosaccharides; prebiotics; transglycosylation; betα-galactosidase; covalent immobilization; Bacillus circulans; glyoxal agarose; fixed-bed reactor
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Rodriguez-Colinas, B.; Fernandez-Arrojo, L.; Santos-Moriano, P.; Ballesteros, A.O.; Plou, F.J. Continuous Packed Bed Reactor with Immobilized β-Galactosidase for Production of Galactooligosaccharides (GOS). Catalysts 2016, 6, 189.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Catalysts EISSN 2073-4344 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top