Catalysts 2012, 2(4), 517-531; doi:10.3390/catal2040517
Article

Advantages of the Biomimetic Nanostructured Films as an Immobilization Method vs. the Carbon Paste Classical Method

1,* email, 2email, 3email and 4email
Received: 27 September 2012; in revised form: 26 October 2012 / Accepted: 1 November 2012 / Published: 9 November 2012
(This article belongs to the Special Issue Biomimetic Catalysts)
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Abstract: Tyrosinase-based biosensors containing a phthalocyanine as electron mediator have been prepared by two different methods. In the first approach, the enzyme and the electron mediator have been immobilized in carbon paste electrodes. In the second method, they have been introduced in an arachidic acid Langmuir-Blodgett nanostructured film that provides a biomimetic environment. The sensing properties of non-nanostructured and nanostructured biosensors towards catechol, catechin and phenol have been analyzed and compared. The enzyme retains the biocatalytic properties in both matrixes. However, the nanostructured biomimetic films show higher values of maximum reaction rates and lowest apparent Michaelis-Menten constants. In both types of sensors, the sensitivity follows the decreasing order catechol > catechin > phenol. The detection limits observed are in the range of 1.8–5.4 μM for Langmuir-Blodgett biosensors and 8.19–8.57 μM for carbon paste biosensors. In summary, it has been demonstrated that the Langmuir-Blodgett films provide a biomimetic environment and nanostructured biosensors show better performances in terms of kinetic, detection limit and stability.
Keywords: bisphthalocyanine; Langmuir-Blodgett; carbon paste; biosensor; tyrosinase
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MDPI and ACS Style

Apetrei, C.; Saja, J.A.; Zurro, J.; Rodríguez-Méndez, M.L. Advantages of the Biomimetic Nanostructured Films as an Immobilization Method vs. the Carbon Paste Classical Method. Catalysts 2012, 2, 517-531.

AMA Style

Apetrei C, Saja JA, Zurro J, Rodríguez-Méndez ML. Advantages of the Biomimetic Nanostructured Films as an Immobilization Method vs. the Carbon Paste Classical Method. Catalysts. 2012; 2(4):517-531.

Chicago/Turabian Style

Apetrei, Constantin; Saja, Jose A.; Zurro, Javier; Rodríguez-Méndez, Maria L. 2012. "Advantages of the Biomimetic Nanostructured Films as an Immobilization Method vs. the Carbon Paste Classical Method." Catalysts 2, no. 4: 517-531.


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