Next Article in Journal
South Asian Medicinal Compounds as Modulators of Resistance to Chemotherapy and Radiotherapy
Previous Article in Journal
Melanoma and the Unfolded Protein Response
Article Menu

Export Article

Open AccessArticle
Cancers 2016, 8(3), 31; doi:10.3390/cancers8030031

Differentially Expressed MicroRNAs in Meningiomas Grades I and II Suggest Shared Biomarkers with Malignant Tumors

1
Department of Medical Biology, Faculty of Health Sciences, UiT-The Arctic University of Norway, NO-9037 Tromsø, Norway
2
Department of Clinical Medicine, Women’s Health and Perinatology Research Group, Faculty of Health Sciences, UiT-The Arctic University of Norway, NO-9037 Tromsø, Norway
3
Department of Obstetrics and Gynecology, University Hospital of North Norway, NO-9038 Tromsø, Norway
4
Department of Medical Genetics, Division of Child and Adolescent Health, University Hospital of North Norway, NO-9038 Tromsø, Norway
5
Department of Pathology, University Hospital of North Norway, NO-9038 Tromsø, Norway
6
Department of Neurosurgery, University Hospital of North Norway, NO-9038 Tromsø, Norway
7
Department of Clinical Medicine, Division of Neurosurgery, Faculty of Health Sciences, UiT-The Arctic University of Norway, NO-9037 Tromsø, Norway
8
Marine Genomics Group, Faculty of Biosciences and Aquaculture, Nord University NO-8049 Bodø, Norway
Current address: Group of Seafood Science, Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, UiT-The Arctic University of Norway, NO-9037 Tromsø, Norway
*
Author to whom correspondence should be addressed.
Academic Editor: Charles Lawrie
Received: 25 January 2016 / Revised: 19 February 2016 / Accepted: 24 February 2016 / Published: 3 March 2016
View Full-Text   |   Download PDF [2904 KB, uploaded 3 March 2016]   |  

Abstract

Meningiomas represent the most common primary tumors of the central nervous system, but few microRNA (miRNA) profiling studies have been reported so far. Deep sequencing of small RNA libraries generated from two human meningioma biopsies WHO grades I (benign) and II (atypical) were compared to excess dura controls. Nineteen differentially expressed miRNAs were validated by RT-qPCR using tumor RNA from 15 patients and 5 meninges controls. Tumor suppressor miR-218 and miR-34a were upregulated relative to normal controls, however, miR-143, miR-193b, miR-451 and oncogenic miR-21 were all downregulated. From 10 selected putative mRNA targets tested by RT-qPCR only four were differentially expressed relative to normal controls. PTEN and E-cadherin (CDH1) were upregulated, but RUNX1T1 was downregulated. Proliferation biomarker p63 was upregulated with nuclear localization, but not detected in most normal arachnoid tissues. Immunoreactivity of E-cadherin was detected in the outermost layer of normal arachnoids, but was expressed throughout the tumors. Nuclear Cyclin D1 expression was positive in all studied meningiomas, while its expression in arachnoid was limited to a few trabecular cells. Meningiomas of grades I and II appear to share biomarkers with malignant tumors, but with some additional tumor suppressor biomarkers expression. Validation in more patients is of importance. View Full-Text
Keywords: meningioma; SOLiD deep sequencing; miRNA; RT-qPCR; cap cells and Immunohistochemistry (IHC) meningioma; SOLiD deep sequencing; miRNA; RT-qPCR; cap cells and Immunohistochemistry (IHC)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

El-Gewely, M.R.; Andreassen, M.; Walquist, M.; Ursvik, A.; Knutsen, E.; Nystad, M.; Coucheron, D.H.; Myrmel, K.S.; Hennig, R.; Johansen, S.D. Differentially Expressed MicroRNAs in Meningiomas Grades I and II Suggest Shared Biomarkers with Malignant Tumors. Cancers 2016, 8, 31.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Cancers EISSN 2072-6694 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top