Next Article in Journal
MET and Small-Cell Lung Cancer
Next Article in Special Issue
Viral Carcinogenesis: Factors Inducing DNA Damage and Virus Integration
Previous Article in Journal
Situational Awareness: Regulation of the Myb Transcription Factor in Differentiation, the Cell Cycle and Oncogenesis
Previous Article in Special Issue
SUMO and KSHV Replication
Article Menu

Export Article

Open AccessArticle
Cancers 2014, 6(4), 2072-2099; doi:10.3390/cancers6042072

From Human Papillomavirus (HPV) Detection to Cervical Cancer Prevention in Clinical Practice

Department of Pathology, Milford Hospital, 300 Seaside Ave., Milford, CT 06460, USA
*
Author to whom correspondence should be addressed.
Received: 4 May 2014 / Revised: 25 September 2014 / Accepted: 25 September 2014 / Published: 2 October 2014
(This article belongs to the Special Issue DNA Viruses in Human Cancer)
View Full-Text   |   Download PDF [1124 KB, uploaded 15 October 2014]   |  

Abstract

The newly gained knowledge of the viral etiology in cervical carcinogenesis has prompted industrial interests in developing virology-based tools for cervical cancer prevention. Due to the long incubation period from viral infection to developing an invasive cancer, a process whose outcome is influenced by numerous life-style and genetic factors, the true efficacy of the genotype-specific human papillomavirus (HPV) vaccines in cervical cancer prevention cannot be determined for another 30 years. Most HPV DNA test kits designed to replace the traditional Papanicolaou (Pap) smears for precancer detection lack the analytical sensitivity and specificity to comprehensively detect all potentially carcinogenic HPVs and to perform reliable genotyping. The authors implemented the classic nested PCR and Sanger DNA-sequencing technology for routine HPV testing. The results showed a true negative HPV PCR invariably indicates the absence of precancerous cells in the cytology samples. However, 80.5% of single positive HPV-16 tests and 97.3% of single positive HPV-18 tests were associated with a negative or a largely self-reversible Pap cytology. Routine sensitive and reliable HPV type-specific or perhaps even variant-specific methods are needed to address the issues of persistence of HPV infection if a virology-based primary cervical screen is used to replace the Pap cytology screening paradigm. View Full-Text
Keywords: reliable HPV genotyping; DNA sequencing; persistent HPV infections; cervical screen; cervical cancer; pap smears; cervical cytology; cervical cancer prevention reliable HPV genotyping; DNA sequencing; persistent HPV infections; cervical screen; cervical cancer; pap smears; cervical cytology; cervical cancer prevention
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Lee, S.H.; Vigliotti, J.S.; Vigliotti, V.S.; Jones, W. From Human Papillomavirus (HPV) Detection to Cervical Cancer Prevention in Clinical Practice. Cancers 2014, 6, 2072-2099.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Cancers EISSN 2072-6694 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top