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Cancers 2014, 6(3), 1570-1578; doi:10.3390/cancers6031570

Simultaneous Expression of Cancer Stem Cell-Like Properties and Cancer-Associated Fibroblast-Like Properties in a Primary Culture of Breast Cancer Cells

1
Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Kobe 650-0047, Japan
2
Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, Kobe 650-0047, Japan
*
Author to whom correspondence should be addressed.
Received: 3 April 2014 / Revised: 16 June 2014 / Accepted: 22 July 2014 / Published: 31 July 2014
(This article belongs to the Special Issue Breast Cancer Biology and Treatment)
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Abstract

The importance of cancer-associated fibroblasts (CAFs) in cancer biology has been recently highlighted owing to their critical roles in cancer growth, progression, metastasis, and therapeutic resistance. We have previously established a primary culture of breast cancer cells, which showed epithelial-mesenchymal transition and cancer stem cell-like properties. In this study, we found that the primary culture also showed CAF-like properties. For example, hypoxia inducible factor 1α (HIF1A) and its downstream genes, nuclear factor-kappa B2 (NF-κB2) and BCL2/adenovirus E1B 19 kd-interacting protein 3 (BNIP3), and many enzymes involved in glycolysis, such as GAPDH, LDH, PGAM1, and PKM2, were highly overexpressed in the primary culture. Moreover, media conditioned with the primary culture cells enhanced the growth of breast cancer cells. Similar to previous CAF studies, this enhancement suggested to be occurred through fibroblast growth factor signaling. This MCKH primary culture cell, which showed simultaneous expression of tumorigenic and CAF properties, offers a unique experimental system for studying the biology of CAFs. View Full-Text
Keywords: cancer-associated fibroblast; cancer stem cell; epithelial-mesenchymal transition; HIF1A; glycolysis cancer-associated fibroblast; cancer stem cell; epithelial-mesenchymal transition; HIF1A; glycolysis
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Ishikawa, M.; Inoue, T.; Shirai, T.; Takamatsu, K.; Kunihiro, S.; Ishii, H.; Nishikata, T. Simultaneous Expression of Cancer Stem Cell-Like Properties and Cancer-Associated Fibroblast-Like Properties in a Primary Culture of Breast Cancer Cells. Cancers 2014, 6, 1570-1578.

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