Cancers 2013, 5(4), 1413-1425; doi:10.3390/cancers5041413
Article

Investigation of siRNA Nanoparticle Formation Using Mono-Cationic Detergents and Its Use in Gene Silencing in Human HeLa Cells

Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 2 September 2013; in revised form: 25 October 2013 / Accepted: 28 October 2013 / Published: 1 November 2013
(This article belongs to the Special Issue Cancers Gene Therapy)
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Abstract: The focus of recent research has been on the development of siRNA vectors to achieve an innovative gene therapy. Most of the conventional vectors are siRNA nanoparticles complexed with cationic polymers and liposomes, making it difficult to release siRNA. In this study, we report on the use of MCD, a quaternary ammonium salt detergent containing a long aliphatic chain (L-chain) as an siRNA complexation agent using human HeLa cells (a model cancer cell). We prepared siRNA nanoparticles using various MCDs, and measured the diameters and zeta-potentials of the particles. The use of an MCD with a long L-chain resulted in the formation of a positively charged nanoparticle. In contrast, a negatively charged nanoparticle was formed when a MCD with a short L-chain was used. We next evaluated the gene silencing efficiency of the nanoparticles using HeLa cells expressing the luciferase protein. The results showed that the siRNA/MCD nanoparticles showed a higher gene silencing efficiency than Lipofectamine 2000. We also found that the efficiency of gene silencing is a function of the length of the alkyl chain in MCD and zeta-potential of the siRNA/MCD nanoparticles. Such information provides another viewpoint for designing siRNA vectors.
Keywords: mono-cationic detergent; gene silencing; siRNA delivery; nanoparticle; multifunctional envelope-type nano device (MEND)

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MDPI and ACS Style

Yamada, Y.; Suzuki, R.; Harashima, H. Investigation of siRNA Nanoparticle Formation Using Mono-Cationic Detergents and Its Use in Gene Silencing in Human HeLa Cells. Cancers 2013, 5, 1413-1425.

AMA Style

Yamada Y, Suzuki R, Harashima H. Investigation of siRNA Nanoparticle Formation Using Mono-Cationic Detergents and Its Use in Gene Silencing in Human HeLa Cells. Cancers. 2013; 5(4):1413-1425.

Chicago/Turabian Style

Yamada, Yuma; Suzuki, Ryosuke; Harashima, Hideyoshi. 2013. "Investigation of siRNA Nanoparticle Formation Using Mono-Cationic Detergents and Its Use in Gene Silencing in Human HeLa Cells." Cancers 5, no. 4: 1413-1425.

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