Ex Vivo Behaviour of Human Bone Tumor Endothelial Cells
Abstract
:1. Introduction
2. Experimental Section
2.1. Human Tissue Samples
2.2. Cytofluorimetric Analysis
2.3. Immunofluorescence Studies
2.4. Nitric Oxide and Platelet Activating Factor (PAF) Assays
2.5. Cell Proliferation Assay
2.6. Tube Formation and Migration Assay
2.7. Statistical Analysis
3. Results
3.1. Characterization of TECs
Case | Gender | Age | Histotype | Grade |
---|---|---|---|---|
1 | M | 30 | GCT * | I |
2 | M | 25 | Chondrosarcoma | II |
3 | M | 40 | Fibrosarcoma | I |
4 | F | 35 | Osteosarcoma | II |
5 | F | 45 | Chondrosarcoma | II |
6 | F | 23 | Chondrosarcoma | II |
7 | M | 43 | Osteosarcoma | III |
8 | F | 31 | Fibrosarcoma | II |
9 | M | 26 | Osteosarcoma | II |
10 | M | 27 | GCT * | I |
11 | F | 22 | Chondrosarcoma | II |
12 | F | 20 | GCT * | I |
13 | F | 45 | GCT * | I |
3.2. In Vitro Behaviour of TECs
4. Discussion
5. Conclusions
References
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Infante, T.; Cesario, E.; Gallo, M.; Fazioli, F.; De Chiara, A.; Tutucci, C.; Apice, G.; De Nigris, F. Ex Vivo Behaviour of Human Bone Tumor Endothelial Cells. Cancers 2013, 5, 404-417. https://doi.org/10.3390/cancers5020404
Infante T, Cesario E, Gallo M, Fazioli F, De Chiara A, Tutucci C, Apice G, De Nigris F. Ex Vivo Behaviour of Human Bone Tumor Endothelial Cells. Cancers. 2013; 5(2):404-417. https://doi.org/10.3390/cancers5020404
Chicago/Turabian StyleInfante, Teresa, Elena Cesario, Michele Gallo, Flavio Fazioli, Annarosaria De Chiara, Cristina Tutucci, Gaetano Apice, and Filomena De Nigris. 2013. "Ex Vivo Behaviour of Human Bone Tumor Endothelial Cells" Cancers 5, no. 2: 404-417. https://doi.org/10.3390/cancers5020404