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Ex Vivo Behaviour of Human Bone Tumor Endothelial Cells
SDN-Foundation, Institute of Diagnostic and Nuclear Development, IRCCS, 80143 Naples, Italy
Department of Biochemistry Biophysics and General Pathology, Second University of Naples, 80138 Naples, Italy
Division of Skeletal Muscles Oncology Surgery, National Cancer Institute, Pascale Foundation, 80131 Naples, Italy
Anatomic Pathology Unit, National Cancer Institute, Pascale Foundation, 80131 Naples, Italy
Medical Oncology of Bone and Soft Sarcoma tissues Unit, National Cancer Institute, Pascale Foundation, 80131 Naples, Italy
* Author to whom correspondence should be addressed.
Received: 19 February 2013; in revised form: 19 March 2013 / Accepted: 3 April 2013 / Published: 11 April 2013
Abstract: Cooperation between endothelial cells and bone in bone remodelling is well established. In contrast, bone microvasculature supporting the growth of primary tumors and metastasis is poorly understood. Several antiangiogenic agents have recently been undergoing trials, although an extensive body of clinical data and experimental research have proved that angiogenic pathways differ in each tumor type and stage. Here, for the first time, we characterize at the molecular and functional level tumor endothelial cells from human bone sarcomas at different stages of disease and with different histotypes. We selected a CD31+ subpopulation from biopsies that displayed the capability to grow as adherent cell lines without vascular endothelial growth factor (VEGF). Our findings show the existence in human primary bone sarcomas of highly proliferative endothelial cells expressing CD31, CD44, CD105, CD146 and CD90 markers. These cells are committed to develop capillary-like structures and colony formation units, and to produce nitric oxide. We believe that a better understanding of tumor vasculature could be a valid tool for the design of an efficacious antiangiogenic therapy as adjuvant treatment of sarcomas.
Keywords: sarcomas; angiogenesis; endothelial cells; tumor microenvironment
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Infante, T.; Cesario, E.; Gallo, M.; Fazioli, F.; De Chiara, A.; Tutucci, C.; Apice, G.; de Nigris, F. Ex Vivo Behaviour of Human Bone Tumor Endothelial Cells. Cancers 2013, 5, 404-417.
Infante T, Cesario E, Gallo M, Fazioli F, De Chiara A, Tutucci C, Apice G, de Nigris F. Ex Vivo Behaviour of Human Bone Tumor Endothelial Cells. Cancers. 2013; 5(2):404-417.
Infante, Teresa; Cesario, Elena; Gallo, Michele; Fazioli, Flavio; De Chiara, Annarosaria; Tutucci, Cristina; Apice, Gaetano; de Nigris, Filomena. 2013. "Ex Vivo Behaviour of Human Bone Tumor Endothelial Cells." Cancers 5, no. 2: 404-417.